Dynamic Transcriptome, Accessible Genome, and PGR Cistrome Profiles in the Human Myometrium

Overview

Journal FASEB J

Specialties Biology
Physiology

Date 2020 Jan 8

PMID 31908010

Citations 10

Authors

San-Pin Wu

Matthew L Anderson

Tianyuan Wang

Lecong Zhou

Olivia M Emery

Xilong Li

Francesco J DeMayo

Affiliations

Soon will be listed here.

Abstract

The myometrium undergoes structural and functional remodeling during pregnancy. We hypothesize that myometrial genomic elements alter correspondingly in preparation for parturition. Human myometrial tissues from nonpregnant (NP) and term pregnant (TP) human subjects were examined by RNAseq, ATACseq, and PGR ChIPseq assays to profile transcriptome, assessible genome, and PGR occupancy. NP and TP specimens exhibit 2890 differentially expressed genes, reflecting an increase of metabolic, inflammatory, and PDGF signaling, among others, in adaptation to pregnancy. At the epigenome level, patterns of accessible genome change between NP and TP myometrium, leading to the altered enrichment of binding motifs for hormone and muscle regulators such as the progesterone receptor (PGR), Krüppel-like factors, and MEF2A transcription factors. PGR genome occupancy exhibits a significant difference between the two stages of the myometrium, concomitant with distinct transcriptomic profiles including genes such as ENO1, LHDA, and PLCL1 in the glycolytic and calcium signaling pathways. Over-representation of SRF, MYOD, and STAT binding motifs in PGR occupying sites further suggests interactions between PGR and major muscle regulators for myometrial gene expression. In conclusion, changes in accessible genome and PGR occupancy are part of the myometrial remodeling process and may serve as mechanisms to formulate the state-specific transcriptome profiles.

Citing Articles

Assessment of the Histone Mark-based Epigenomic Landscape in Human Myometrium at Term Pregnancy.

Wu S, Quiroz E, Wang T, Redecke S, Xu X, Lin L bioRxiv. 2025; .

PMID: 40060655 PMC: 11888205. DOI: 10.1101/2024.02.19.581035.

The myometrial transcriptome changes in mares with endometrosis.

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KDM6B-dependent epigenetic programming of uterine fibroblasts in early pregnancy regulates parturition timing in mice.

McIntyre T, Valdez O, Kochhar N, Davidson B, Samad B, Qiu L Cell. 2025; 188(5):1265-1279.e18.

PMID: 39842437 PMC: 11890963. DOI: 10.1016/j.cell.2024.12.019.

Genome-Wide Association Analysis of Growth Traits in Hu Sheep.

Li T, Xing F, Zhang N, Chen J, Zhang Y, Yang H Genes (Basel). 2025; 15(12.

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The hormonal control of parturition.

Hamburg-Shields E, Mesiano S Physiol Rev. 2024; 104(3):1121-1145.

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