Inflammasome Activation Induced by a Snake Venom Lys49-Phospholipase A Homologue

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2020 Jan 8

PMID 31906173

Citations 5

Authors

Charles Nunes Boeno

Mauro Valentino Paloschi

Jessica Amaral Lopes

Weverson Luciano Pires

Sulamita da Silva Setubal

Jaina Rodrigues Evangelista

Andreimar Martins Soares

Juliana Pavan Zuliani

Affiliations

Soon will be listed here.

Abstract

Background: Snake venom phospholipases A (PLAs) have hemolytic, anticoagulant, myotoxic, oedematogenic, bactericidal, and inflammatory actions. BthTX-I, a Lys49-PLA isolated from venom, is an example of Lys49-PLA that presents such actions. NLRP3 is a cytosolic receptor from the NLR family responsible for inflammasome activation via caspase-1 activation and IL-1β liberation. The study of NLRs that recognize tissue damage and activate the inflammasome is relevant in envenomation.

Methods: Male mice (18-20 g) received an intramuscular injection of BthTX-I or sterile saline. The serum was collected for creatine-kinase (CK), lactate dehydrogenase (LDH), and interleukin-1β (IL-1β) assays, and muscle was removed for inflammasome activation immunoblotting and qRT-PCR expression for nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin-containing domain 3 receptor (NLRP3) inflammasome components.

Results: BthTX-I-induced inflammation and myonecrosis, shown by intravital microscope, and LDH and CK release, respectively. Mouse treatment with A438079, a P2X7 receptor antagonist, did not modify these effects. BthTX-I induced inflammasome activation in muscle, but P2X7R participation in this effect was not observed.

Conclusion: Together, the results showed for the first time that BthTX-I in gastrocnemius muscle induces inflammation and consequently, inflammasome activation via NLRP3 with caspase-1 activation and IL-1β liberation.

Citing Articles

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