Effects of the Entomopathogenic Fungus Metarhizium Flavoviride on the Fat Body Lipid Composition of Zophobas Morio Larvae (Coleoptera: Tenebrionidae)

Overview

Journal Naturwissenschaften

Specialty Science

Date 2020 Jan 5

PMID 31900598

Citations 6

Authors

Marek Golebiowski

Aleksandra Urbanek

Anna Pietrzak

Aleksandra M Naczk

Aleksandra Bojke

Cezary Tkaczuk

Piotr Stepnowski

Affiliations

Soon will be listed here.

Abstract

Insects employ different defense strategies against fungal infections and chemicals. We aimed to identify the lipid compositions of the fat body of Zophobas morio larvae before and after fungal infection with the entomopathogenic fungus Metarhizium flavoviride. We used gas chromatography-mass spectrometry to analyze lipid extracts of the fat body isolated of Z. morio 2, 5, and 7 days after fungal infection (treatment group) and compared it with the lipid extracts in a control group injected with physiological isotonic saline. In all the samples, fatty acids were the most abundant compound found in the fat body extracts, with hexadecanoic acid/C16:0 being the most abundant lipid. However, the types and concentrations of lipids changed after fungal infection, likely as an immune response. The most considerable changes occurred in the concentrations of long-chain fatty acids, i.e., hexadecanoic acid/C16:0, octadecenoic acid/C18:1, and octadecanoic acid/C18:0. Contents of methyl ester increased significantly after infection, but that of other esters, especially octanoic acid decyl ester/OADE, decreased on the 5th day after infection. To the best of our knowledge, this is the first detailed analysis of the changes in the lipid composition of the fat body of Z. morio larvae as a result of fungal infection. Our results suggest that entomopathogenic fungal infection affects the internal lipid composition of insects, potentially as a way of adjusting to such infection. These results can help understand infection processes and defense strategies of insects against fungal infection. Ultimately, they can contribute to the creation of more effective chemicals against pest insects.

Citing Articles

Transcriptome and Metabolome Analyses of in Response to Infection.

Li M, Zhang J, Qin Q, Zhang H, Li X, Wang H Microorganisms. 2023; 11(9).

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Lipids as a key element of insect defense systems.

Wronska A, Kaczmarek A, Bogus M, Kuna A Front Genet. 2023; 14:1183659.

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The metabolism and role of free fatty acids in key physiological processes in insects of medical, veterinary and forensic importance.

Kaczmarek A, Bogus M PeerJ. 2022; 9:e12563.

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References

Schal C, Gu X, Burns E, Blomquist G . Patterns of biosynthesis and accumulation of hydrocarbons and contact sex pheromone in the female German cockroach, Blattella germanica. Arch Insect Biochem Physiol. 1994; 25(4):375-91. DOI: 10.1002/arch.940250411. View

Zheng C, Yoo J, Lee T, Cho H, Kim Y, Kim W . Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids. FEBS Lett. 2005; 579(23):5157-62. DOI: 10.1016/j.febslet.2005.08.028. View

Golebiowski M, Cerkowniak M, Urbanek A, Dawgul M, Kamysz W, Bogus M . Identification and antifungal activity of novel organic compounds found in cuticular and internal lipids of medically important flies. Microbiol Res. 2014; 170:213-22. DOI: 10.1016/j.micres.2014.06.004. View

Kostal V, Urban T, rimnacova L, Berkova P, Simek P . Seasonal changes in minor membrane phospholipid classes, sterols and tocopherols in overwintering insect, Pyrrhocoris apterus. J Insect Physiol. 2013; 59(9):934-41. DOI: 10.1016/j.jinsphys.2013.06.008. View

Kuhnlein R . Thematic review series: Lipid droplet synthesis and metabolism: from yeast to man. Lipid droplet-based storage fat metabolism in Drosophila. J Lipid Res. 2012; 53(8):1430-6. PMC: 3540836. DOI: 10.1194/jlr.R024299. View

Svoboda J . Variability of metabolism and function of sterols in insects. Crit Rev Biochem Mol Biol. 1999; 34(1):49-57. DOI: 10.1080/10409239991209183. View

Bogus M, Kedra E, Bania J, Szczepanik M, Czygier M, Jablonski P . Different defense strategies of Dendrolimus pini, Galleria mellonella, and Calliphora vicina against fungal infection. J Insect Physiol. 2007; 53(9):909-22. DOI: 10.1016/j.jinsphys.2007.02.016. View

Keyser C, Licht H, Steinwender B, Meyling N . Diversity within the entomopathogenic fungal species Metarhizium flavoviride associated with agricultural crops in Denmark. BMC Microbiol. 2015; 15:249. PMC: 4628438. DOI: 10.1186/s12866-015-0589-z. View

Golebiowski M, Urbanek A, Oleszczak A, Dawgul M, Kamysz W, Bogus M . The antifungal activity of fatty acids of all stages of Sarcophaga carnaria L. (Diptera: Sarcophagidae). Microbiol Res. 2013; 169(4):279-86. DOI: 10.1016/j.micres.2013.07.011. View

10.

Roberts D, St Leger R . Metarhizium spp., cosmopolitan insect-pathogenic fungi: mycological aspects. Adv Appl Microbiol. 2004; 54:1-70. DOI: 10.1016/S0065-2164(04)54001-7. View

11.

Canavoso L, Jouni Z, Karnas K, Pennington J, Wells M . Fat metabolism in insects. Annu Rev Nutr. 2001; 21:23-46. DOI: 10.1146/annurev.nutr.21.1.23. View

12.

Cuthbertson A, Walters K, Deppe C . Compatibility of the entomopathogenic fungus Lecanicillium muscarium and insecticides for eradication of sweetpotato whitefly, Bemisia tabaci. Mycopathologia. 2005; 160(1):35-41. DOI: 10.1007/s11046-005-6835-4. View

13.

Samuels R, Paterson I . Cuticle degrading proteases from insect moulting fluid and culture filtrates of entomopathogenic fungi. Comp Biochem Physiol B Biochem Mol Biol. 1995; 110(4):661-9. DOI: 10.1016/0305-0491(94)00205-9. View

14.

Golebiowski M, Cerkowniak M, Bogus M, Wloka E, Dawgul M, Kamysz W . Free fatty acids in the cuticular and internal lipids of Calliphora vomitoria and their antimicrobial activity. J Insect Physiol. 2013; 59(4):416-29. DOI: 10.1016/j.jinsphys.2013.02.001. View

15.

Cheon H, Shin S, Bian G, Park J, Raikhel A . Regulation of lipid metabolism genes, lipid carrier protein lipophorin, and its receptor during immune challenge in the mosquito Aedes aegypti. J Biol Chem. 2006; 281(13):8426-35. DOI: 10.1074/jbc.M510957200. View

16.

Hoffmann J . Innate immunity of insects. Curr Opin Immunol. 1995; 7(1):4-10. DOI: 10.1016/0952-7915(95)80022-0. View

17.

Cerkowniak M, Puckowski A, Stepnowski P, Golebiowski M . The use of chromatographic techniques for the separation and the identification of insect lipids. J Chromatogr B Analyt Technol Biomed Life Sci. 2013; 937:67-78. DOI: 10.1016/j.jchromb.2013.08.023. View

18.

Urbanek A, Szadziewski R, Stepnowski P, Boros-Majewska J, Gabriel I, Dawgul M . Composition and antimicrobial activity of fatty acids detected in the hygroscopic secretion collected from the secretory setae of larvae of the biting midge Forcipomyia nigra (Diptera: Ceratopogonidae). J Insect Physiol. 2012; 58(9):1265-76. DOI: 10.1016/j.jinsphys.2012.06.014. View

19.

Shah P, Pell J . Entomopathogenic fungi as biological control agents. Appl Microbiol Biotechnol. 2003; 61(5-6):413-23. DOI: 10.1007/s00253-003-1240-8. View

20.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View