Integration of Hepatitis B Virus DNA into P21-activated Kinase 3 (PAK3) Gene in HepG2.2.15 Cells

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2020 Jan 4

PMID 31897927

Citations 7

Authors

Peng Ruan

Xiufang Dai

Jun Sun

Chunping He

Chao Huang

Rui Zhou

Isabelle Chemin

Affiliations

Soon will be listed here.

Abstract

Integration of HBV DNA into host chromosomes was found in most of the patients with chronic hepatitis B (CHB). In this study, using inverse nested PCR (invPCR), we found the integration site chrX: 111,009,033, which inserted into the p21-activated kinase 3 (PAK3) gene in HepG2.2.15 cells. The viral-human chimeric transcripts were also observed and, significant differences of the copy numbers of integration site chrX: 111,009,033 (P = 0.012) and intra-cell HBV DNA levels (P = 0.027) were found between the cells with and without HO treatment, respectively. This study may provide a novel insight into the elucidation of etiology of HBV integration.

Citing Articles

The impact of integrated hepatitis B virus DNA on oncogenesis and antiviral therapy.

Zhang M, Chen H, Liu H, Tang H Biomark Res. 2024; 12(1):84.

PMID: 39148134 PMC: 11328401. DOI: 10.1186/s40364-024-00611-y.

Two fragments of HBV DNA integrated into chrX: 11009033 and its genetic regulation in HepG2.2.15.

Ruan P, Zhou R, He C, Huang C, Lin M, Yin H Mol Med Rep. 2023; 27(5).

PMID: 36960866 PMC: 10086561. DOI: 10.3892/mmr.2023.12985.

P-21 Activated Kinases in Liver Disorders.

Qiu X, Xu H, Wang K, Gao F, Xu X, He H Cancers (Basel). 2023; 15(2).

PMID: 36672500 PMC: 9857091. DOI: 10.3390/cancers15020551.

Insights into Hepatitis B Virus DNA Integration-55 Years after Virus Discovery.

Zhao K, Liu A, Xia Y Innovation (Camb). 2021; 1(2):100034.

PMID: 34557710 PMC: 8454683. DOI: 10.1016/j.xinn.2020.100034.

Targeted Long-Read Sequencing Reveals Comprehensive Architecture, Burden, and Transcriptional Signatures from Hepatitis B Virus-Associated Integrations and Translocations in Hepatocellular Carcinoma Cell Lines.

Ramirez R, van Buuren N, Gamelin L, Soulette C, May L, Han D J Virol. 2021; 95(19):e0029921.

PMID: 34287049 PMC: 8428387. DOI: 10.1128/JVI.00299-21.

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