Molecular Characterization of Chicken Astroviruses in Gout-affected Commercial Broiler Chickens in Haryana, India

Overview

Journal Virusdisease

Publisher Springer

Specialty Microbiology

Date 2020 Jan 4

PMID 31897418

Citations 6

Authors

Sumitra Panigrahi

Naresh Jindal

Pawan Kumar

Sanjay Barua

Naveen Kumar

Thachamvally Riyesh

Yogesh Chander

Affiliations

Soon will be listed here.

Abstract

Chicken astroviruses (CAstVs) infect young chicks and are associated with gastroenteritis, stunted growth or visceral gout (gout). True incidence and distribution of CAstVs as well as virus variants circulating in India is not well understood. In this study, 80 gout-affected broiler chicken flocks from Haryana, a north-western state of India, were tested for the presence of astroviruses by targeting the polymerase gene of both CAstV and avian nephritis virus (ANV) and capsid gene of CAstV. Of these, 22 (27.5%) flocks were found positive for CAstV, 7(8.75%) for ANV and 2 (2.5%) for both CAstV and ANV genome by reverse-transcription-polymerase chain reaction. CAstV was isolated by inoculating tissue (kidney) homogenate from gout-affected birds into specific-pathogen free embryonated chicken eggs where the infected embryos showed stunted growth with necrosis of liver and enlarged kidney with urate deposits. Capsid gene-based phylogenetic analysis revealed the clustering of CAstV strains from this study with Indian strains of serogroup Biii suggesting their antigenic relatedness. Thus the present study reveals the presence of chicken astroviruses in broiler chickens affected with gout.

Citing Articles

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References

Pantin-Jackwood M, Spackman E, Woolcock P . Molecular characterization and typing of chicken and turkey astroviruses circulating in the United States: implications for diagnostics. Avian Dis. 2006; 50(3):397-404. DOI: 10.1637/7512-020606R.1. View

Patel A, Pandit R, Thakkar J, Hinsu A, Pandey V, Pal J . Complete genome sequence analysis of chicken astrovirus isolate from India. Vet Res Commun. 2016; 41(1):67-75. PMC: 7088555. DOI: 10.1007/s11259-016-9673-6. View

Kaithal B, Jindal N, Kumar P, Mor S . Detection and molecular characterization of enteric viruses in enteritis-affected commercial broiler chickens in India. Acta Virol. 2016; 60(4):361-371. DOI: 10.4149/av_2016_04_361. View

De la Torre D, Nunez L, Astolfi-Ferreira C, Ferreira A . Enteric Virus Diversity Examined by Molecular Methods in Brazilian Poultry Flocks. Vet Sci. 2018; 5(2). PMC: 6024555. DOI: 10.3390/vetsci5020038. View

Hewson K, ORourke D, Noormohammadi A . Detection of avian nephritis virus in Australian chicken flocks. Avian Dis. 2010; 54(3):990-3. DOI: 10.1637/9230-010610-Reg.1. View

Day J, Spackman E, Pantin-Jackwood M . A multiplex RT-PCR test for the differential identification of turkey astrovirus type 1, turkey astrovirus type 2, chicken astrovirus, avian nephritis virus, and avian rotavirus. Avian Dis. 2007; 51(3):681-4. DOI: 10.1637/0005-2086(2007)51[681:AMRTFT]2.0.CO;2. View

Devaney R, Trudgett J, Trudgett A, Meharg C, Smyth V . A metagenomic comparison of endemic viruses from broiler chickens with runting-stunting syndrome and from normal birds. Avian Pathol. 2016; 45(6):616-629. PMC: 7113909. DOI: 10.1080/03079457.2016.1193123. View

Mandoki M, Bakonyi T, Ivanics E, Nemes C, Dobos-Kovacs M, Rusvai M . Phylogenetic diversity of avian nephritis virus in Hungarian chicken flocks. Avian Pathol. 2006; 35(3):224-9. DOI: 10.1080/03079450600711037. View

Smyth V, Todd D, Trudgett J, Lee A, Welsh M . Capsid protein sequence diversity of chicken astrovirus. Avian Pathol. 2012; 41(2):151-9. DOI: 10.1080/03079457.2011.652938. View

10.

Baxendale W, Mebatsion T . The isolation and characterisation of astroviruses from chickens. Avian Pathol. 2004; 33(3):364-70. DOI: 10.1080/0307945042000220426. View

11.

Long K, Ouckama R, Weisz A, Brash M, Ojkic D . White Chick Syndrome Associated with Chicken Astrovirus in Ontario, Canada. Avian Dis. 2018; 62(2):247-258. DOI: 10.1637/11802-012018-Case.1. View

12.

Nunez L, Parra S, Mettifogo E, Catroxo M, Astolfi-Ferreira C, Ferreira A . Isolation of chicken astrovirus from specific pathogen-free chicken embryonated eggs. Poult Sci. 2015; 94(5):947-54. DOI: 10.3382/ps/pev086. View

13.

Kang K, Linnemann E, Icard A, Durairaj V, Mundt E, Sellers H . Chicken astrovirus as an aetiological agent of runting-stunting syndrome in broiler chickens. J Gen Virol. 2018; 99(4):512-524. DOI: 10.1099/jgv.0.001025. View

14.

Mettifogo E, Nunez L, Chacon J, Parra S, Astolfi-Ferreira C, Jerez J . Emergence of enteric viruses in production chickens is a concern for avian health. ScientificWorldJournal. 2014; 2014:450423. PMC: 3919086. DOI: 10.1155/2014/450423. View

15.

Pantin-Jackwood M, Strother K, Mundt E, Zsak L, Day J, Spackman E . Molecular characterization of avian astroviruses. Arch Virol. 2010; 156(2):235-44. DOI: 10.1007/s00705-010-0849-z. View

16.

Imada T, Yamaguchi S, Mase M, Tsukamoto K, Kubo M, Morooka A . Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA. J Virol. 2000; 74(18):8487-93. PMC: 116360. DOI: 10.1128/jvi.74.18.8487-8493.2000. View

17.

Bayry J, Goudar M, Nighot P, Kshirsagar S, Ladman B, Gelb Jr J . Emergence of a nephropathogenic avian infectious bronchitis virus with a novel genotype in India. J Clin Microbiol. 2005; 43(2):916-8. PMC: 548090. DOI: 10.1128/JCM.43.2.916-918.2005. View

18.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

19.

Jindal N, Patnayak D, Chander Y, Ziegler A, Goyal S . Detection and molecular characterization of enteric viruses from poult enteritis syndrome in turkeys. Poult Sci. 2010; 89(2):217-26. PMC: 7107190. DOI: 10.3382/ps.2009-00424. View

20.

Bulbule N, Mandakhalikar K, Kapgate S, Deshmukh V, Schat K, Chawak M . Role of chicken astrovirus as a causative agent of gout in commercial broilers in India. Avian Pathol. 2013; 42(5):464-73. DOI: 10.1080/03079457.2013.828194. View