Selection of Peptides and Proteins-Advantages of MRNA Display

Overview

Journal ACS Synth Biol

Publisher American Chemical Society

Specialties Biotechnology
Molecular Biology

Date 2020 Jan 1

PMID 31891492

Citations 34

Authors

Matilda S Newton

Yari Cabezas-Perusse

Cher Ling Tong

Burckhard Seelig

Affiliations

Soon will be listed here.

Abstract

mRNA display is a robust selection technique that allows the selection of peptides and proteins with desired functions from libraries of trillions of variants. mRNA display relies upon a covalent linkage between a protein and its encoding mRNA molecule; the power of the technique stems from the stability of this link, and the large degree of control over experimental conditions afforded to the researcher. This article describes the major advantages that make mRNA display the method of choice among comparable and methods, including cell-surface display, phage display, and ribosomal display. We also describe innovative techniques that harness mRNA display for directed evolution, protein engineering, and drug discovery.

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