Evolution of Microbial Community During Dry Storage and Recovery of Aerobic Granular Sludge

Overview

Journal Heliyon

Specialty Social Sciences

Date 2020 Jan 1

PMID 31890963

Citations 1

Authors

Linan Zhang

Bei Long

Junfeng Wu

Yuanyuan Cheng

Binchao Zhang

Yu Zeng

Sinong Huang

Mingjing Zeng

Affiliations

Soon will be listed here.

Abstract

Aerobic granular sludge (AGS) was imbedded in agar and stored at 4 °C for 30 days, and then the stored granules were recovered in a sequencing batch reactor fed real wastewater within 11 days. Variations in microbial community compositions were investigated during dry storage and recovery of AGS, aiming to elucidate the mechanism of granular stability loss and recovery. The storage and recovery of AGS involved microbial community evolution. The dominant bacterial genera of the mature AGS were (relative abundance of 22.39%), (16.03%) and (11.17%), and those of the stored granules were (26.79%), (12.83%) and (5.69%), respectively. However, the dominant genera were (43.64%), (12.3.6%) and (11.47%) in the recovered AGS. Methanogens were always the dominant archaeal species in mature AGS (93.01%), stored granules (99.99%) and the recovered AGS (94.84%). Facultative anaerobes and anaerobes proliferated and dominated in the stored granules, and their metabolic activities gradually led to granular structure destruction and property deterioration. However, the stored granules served as carriers for the microbes originated from the real septic tank wastewater during recovery. They proliferated rapidly and secreted a large number of extracellular polymeric substances which helped to recover the granular structure in 11 days.

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PMID: 37842668 PMC: 10573856. DOI: 10.1039/d3ra05371g.

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