Proteomic Analyses of Decellularized Porcine Ovaries Identified New Matrisome Proteins and Spatial Differences Across and Within Ovarian Compartments

Overview

Journal Sci Rep

Specialty Science

Date 2019 Dec 29

PMID 31882863

Citations 29

Authors

Nathaniel F Henning

Richard D LeDuc

Kelly A Even

Monica M Laronda

Affiliations

Soon will be listed here.

Abstract

Premature ovarian insufficiency (POI) affects approximately 1% of women. We aim to understand the ovarian microenvironment, including the extracellular matrix (ECM) and associated proteins (matrisome), and its role in controlling folliculogenesis. We mapped the composition of the matrisome of porcine ovaries through the cortical compartment, where quiescent follicles reside and the medullary compartment, where the larger follicles grow and mature. To do this we sliced the ovaries, uniformly in two anatomical planes, enriched for matrisome proteins and performed bottom-up shotgun proteomic analyses. We identified 42 matrisome proteins that were significantly differentially expressed across depths, and 11 matrisome proteins that have not been identified in previous ovarian protein analyses. We validated these data for nine proteins and confirmed compartmental differences with a second processing method. Here we describe a processing and proteomic analysis pipeline that revealed spatial differences and matrisome protein candidates that may influence folliculogenesis.

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