Transcriptome-wide Analysis of the SCNT Bovine Abnormal Placenta During Mid- to Late Gestation

Overview

Journal Sci Rep

Specialty Science

Date 2019 Dec 29

PMID 31882783

Citations 5

Authors

Guangqi Gao

Shenyuan Wang

Jiaqi Zhang

Guanghua Su

Zhong Zheng

Chunling Bai

Lei Yang

Zhuying Wei

Xiuying Wang

Xiao Liu

Ziru Guo

Guangpeng Li

Xiaohu Su

Li Zhang

Affiliations

Soon will be listed here.

Abstract

The dysfunction of placenta is common in somatic cell nuclear transfer (SCNT) cloned cattle and would cause aberrant fetal development and even abortion, which occurred with highest rate at the mid- to late gestation. However, the mechanism of abnormal placentas was unclear. To analyze the transcriptome-wide characteristics of abnormal placentas in SCNT cloned cattle, the mRNA, lncRNA and miRNA of placental cotyledon tissue at day 180 after gestation were sequenced. A total of 19,055 mRNAs, 30,141 lncRNAs and 684 miRNAs were identified. Compared with control group, 362 mRNAs, 1,272 lncRNAs and nine miRNAs (six known and three novel miRNAs) were differentially expressed (fold change ≥ 2 and P-value < 0.05). The differentially expressed genes were functionally enriched in urea and ions transmembrane transport, which indicated that the maternal-fetal interactions were disturbed in impaired placentas. Furthermore, the competing endogenous RNAs (ceRNAs) networks were identified to illustrate their roles in abnormal placental morphology. The present research would be helpful to discover the mechanism of late gestational abnormality of SCNT cattle by provides important genomic information and insights.

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