Detection of Serum MiRNAs Affecting Liver Apoptosis in a Periodontitis Rat Model

Overview

Journal In Vivo

Specialty Oncology

Date 2019 Dec 29

PMID 31882470

Citations 3

Authors

Yoshio Sugiura

Toshiki Yoneda

Kohei Fujimori

Takayuki Maruyama

Hisataka Miyai

Terumasa Kobayashi

Daisuke Ekuni

Takaaki Tomofuji

Manabu Morita

Affiliations

Soon will be listed here.

Abstract

Background/aim: miRNA molecules have been attracting attention as genetic modifiers between organs. We examined the relationship between serum miRNA and targeted liver mRNA profiles in a periodontitis rat model, and the influence of periodontitis on the liver.

Materials And Methods: Male Wistar rats (n=16, 8 weeks old) were randomly divided into two groups (8 rats each): control and periodontitis (ligature placement for 4 weeks). Serum miRNA and liver mRNA profiles were compared.

Results: Periodontal destruction and hepatocyte apoptosis were induced in the periodontitis group. Microarray analysis indicated that 52 serum miRNAs and 33 liver mRNAs were expressed with a >1.5-fold change (FC) and a >2.0-FC (p<0.05), respectively, between the two groups. From the miRNA target genes, 12 genes equivalented to liver mRNAs with a >2.0-FC, among which, Hyou1, Chac1, and Bloc1s3 have apoptotic functions in our model. miRNAs upstream of these 3 mRNAs are miR-3591, miR-181a-2-3p and miR-6321.

Conclusion: miR-3591, miR-181a-2-3p and miR-6321 induced hepatocyte apoptosis in our periodontitis rat model.

Citing Articles

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