Characteristics of Strains Isolated from Milk and Humans and the Possibility of Milk-Borne Strains Transmission

Overview

Journal Pol J Microbiol

Specialty Microbiology

Date 2019 Dec 28

PMID 31880881

Citations 3

Authors

Krzysztof Skowron

Ewa Walecka-Zacharksa

Katarzyna Grudlewska

Natalia Wiktorczyk

Agnieszka Kaczmarek

Grzegorz Gryn

Joanna Kwiecinska-Pirog

Klaudia Juszczuk

Zbigniew Paluszak

Katarzyna Kosek-Paszkowska

Eugenia Gospodarek-Komkowska

Affiliations

Soon will be listed here.

Abstract

is the etiological factor of listeriosis. The main source of these organisms is food, including dairy products. The aim was to determine the multiple correlations between the drug susceptibility, virulence genes (VGs), and biofilm formation on silicone teat cups of milk-borne and human strains. The spread of via contaminated teat rubbers was assessed. The strains recovered from milk (18), human blood (10), and the reference strain ATCC®19111™ were used in the study. Penicillin resistance was the most prevalent resistance in the milk isolates (n=8; 44.4%), whereas among clinical strains erythromycin resistance was predominating - (n=6; 60%). The most frequent VGs among strains isolated from milk were (100%) and (100%) whereas in strains isolated from blood - (100%) and (90%). All tested VGs were present in 50% of blood isolates and 11% of milk-borne strains. The strains isolated from milk formed a significantly stronger biofilm. The strains with more numerous virulence genes were resistant to more antibiotics and formed a stronger biofilm. It was shown that contaminated teat cups might contribute to the transmission of in the herd. It seems reasonable to monitor the occurrence of biofilm in a dairy processing environment. is the etiological factor of listeriosis. The main source of these organisms is food, including dairy products. The aim was to determine the multiple correlations between the drug susceptibility, virulence genes (VGs), and biofilm formation on silicone teat cups of milk-borne and human strains. The spread of via contaminated teat rubbers was assessed. The strains recovered from milk (18), human blood (10), and the reference strain ATCC®19111™ were used in the study. Penicillin resistance was the most prevalent resistance in the milk isolates (n=8; 44.4%), whereas among clinical strains erythromycin resistance was predominating – (n=6; 60%). The most frequent VGs among strains isolated from milk were (100%) and (100%) whereas in strains isolated from blood – (100%) and (90%). All tested VGs were present in 50% of blood isolates and 11% of milk-borne strains. The strains isolated from milk formed a significantly stronger biofilm. The strains with more numerous virulence genes were resistant to more antibiotics and formed a stronger biofilm. It was shown that contaminated teat cups might contribute to the transmission of in the herd. It seems reasonable to monitor the occurrence of biofilm in a dairy processing environment.

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