Supplementation of Culture Media with Lysophosphatidic Acid Improves The Follicular Development of Human Ovarian Tissue After Xenotransplantaion into The Back Muscle of γ-Irradiated Mice

Overview

Journal Cell J

Specialty Cell Biology

Date 2019 Dec 22

PMID 31863662

Citations 2

Authors

Zeynab Mohammadi

Nasim Hayati Roodbari

Kazem Parivar

Mojdeh Salehnia

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of the present study was to evaluate the effects of lysophosphatidic acid (LPA) supplementation of human ovarian tissue culture media on tissue survival, follicular development and expression of apoptotic genes following xenotransplantation.

Materials And Methods: In this experimental study, human ovarian tissue was collected from eight normal female to male transsexual individuals and cut into small fragments. These fragments were vitrified-warmed and cultured for 24 hours in the presence or absence of LPA, then xenografted into back muscles of γ-irradiated mice. Two weeks post-transplantation the morphology of the recovered tissues were evaluated by hematoxylin and eosin staining. The expression of genes related to apoptosis ( and ) were analyzed by real time revers transcription polymerase chain reaction (RT-PCR) and detection of BAX protein was done by immunohistochemical staining.

Results: The percent of normal and growing follicles were significantly increased in both grafted groups in comparison to the non-grafted groups, however, these rates were higher in the LPA-treated group than the non-treated group (P<0.05). There was a higher expression of the anti-apoptotic gene, BCL2, but a lower expression of the pro-apoptotic gene, BAX, and a significant lower BAX/ BCL2 ratio in the LPA-treated group in comparison with non-treated control group (P<0.05). No immunostaining positive cells for BAX were observed in the follicles and oocytes in both transplanted ovarian groups.

Conclusion: Supplementation of human ovarian tissue culture medium with LPA improves follicular survival and development by promoting an anti-apoptotic balance in transcription of and genes.

Citing Articles

Lysophosphatidic acid supports the development of vitrified ovarian follicles by decreasing the incidence of cell death: An experimental study.

Abedpour N, Ghorbanmehr N, Salehnia M Int J Reprod Biomed. 2022; 20(4):273-288.

PMID: 35822184 PMC: 9260067. DOI: 10.18502/ijrm.v20i4.10899.

Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models.

Pimentel M, Dos Santos F, de Macedo L, de Brito P, Lima G, Barreto R Anim Reprod. 2020; 17(2):e20190115.

PMID: 32714451 PMC: 7375861. DOI: 10.1590/1984-3143-AR2019-0115.

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