Nitric Oxide Improves the Tolerance of Pleurotus Ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2019 Dec 22

PMID 31862720

Citations 10

Authors

Ludan Hou

Mengran Zhao

Chenyang Huang

Xiangli Wu

Jinxia Zhang

Affiliations

Soon will be listed here.

Abstract

is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO alleviated oxidative damage to mycelia by inhibiting the protein and gene expression of aconitase (ACO), and additional studies found that the overexpression and interference of could affect the content of citric acid (CA). Furthermore, the addition of exogenous CA can induce alternative oxidase () gene expression under heat stress, reduce the content of HO in mycelium, and consequently protect the mycelia under heat stress. An additional analysis focused on the function of the gene in the heat stress response of mycelia. The results show that the colony diameter of the overexpression (OE-) strains was significantly larger than that of the wild-type (WT) strain under heat stress (32°C). In addition, the mycelia of OE- strains showed significantly enhanced tolerance to HO In conclusion, this study demonstrates that NO can affect CA accumulation by regulating gene and ACO protein expression and that CA can induce gene expression and thereby be a response to heat stress. Heat stress is one of the abiotic stresses that affect the growth and development of edible fungi. Our previous study found that exogenous NO had a protective effect on mycelia under heat stress. However, its regulatory mechanism had not been elucidated. In this study, we found that NO altered the respiratory pathway of mycelia under heat stress by regulating The results have enhanced our understanding of NO signaling pathways in .

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References

Napoli C, Paolisso G, Casamassimi A, Al-Omran M, Barbieri M, Sommese L . Effects of nitric oxide on cell proliferation: novel insights. J Am Coll Cardiol. 2013; 62(2):89-95. DOI: 10.1016/j.jacc.2013.03.070. View

Lei M, Wu X, Huang C, Qiu Z, Wang L, Zhang R . Trehalose induced by reactive oxygen species relieved the radial growth defects of Pleurotus ostreatus under heat stress. Appl Microbiol Biotechnol. 2019; 103(13):5379-5390. DOI: 10.1007/s00253-019-09834-8. View

Zhang Z, Luo S, Zhang G, Feng L, Zheng C, Zhou Y . Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017; 40(9):1834-1848. DOI: 10.1111/pce.12989. View

Baidya S, Cary J, Grayburn W, Calvo A . Role of nitric oxide and flavohemoglobin homolog genes in Aspergillus nidulans sexual development and mycotoxin production. Appl Environ Microbiol. 2011; 77(15):5524-8. PMC: 3147476. DOI: 10.1128/AEM.00638-11. View

Feng H, Li H, Sun K . Enhanced expression of alternative oxidase genes is involved in the tolerance of rice (Oryza sativa L.) seedlings to drought stress. Z Naturforsch C J Biosci. 2009; 64(9-10):704-10. DOI: 10.1515/znc-2009-9-1016. View

Zidenga T, Leyva-Guerrero E, Moon H, Siritunga D, Sayre R . Extending cassava root shelf life via reduction of reactive oxygen species production. Plant Physiol. 2012; 159(4):1396-407. PMC: 3425186. DOI: 10.1104/pp.112.200345. View

Kong W, Huang C, Chen Q, Zou Y, Zhang J . Nitric oxide alleviates heat stress-induced oxidative damage in Pleurotus eryngii var. tuoliensis. Fungal Genet Biol. 2011; 49(1):15-20. DOI: 10.1016/j.fgb.2011.12.003. View

Liu Y, Jiang H, Zhao Z, An L . Nitric oxide synthase like activity-dependent nitric oxide production protects against chilling-induced oxidative damage in Chorispora bungeana suspension cultured cells. Plant Physiol Biochem. 2010; 48(12):936-44. DOI: 10.1016/j.plaphy.2010.09.001. View

Zou Y, Zhang M, Qu J, Zhang J . iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of in Response to Heat Stress and Subsequent Recovery. Front Microbiol. 2018; 9:2368. PMC: 6189471. DOI: 10.3389/fmicb.2018.02368. View

10.

Liu Y, Lu X, Ren A, Shi L, Zhu J, Jiang A . Conversion of phosphatidylinositol (PI) to PI4-phosphate (PI4P) and then to PI(4,5)P is essential for the cytosolic Ca concentration under heat stress in Ganoderma lucidum. Environ Microbiol. 2018; 20(7):2456-2468. DOI: 10.1111/1462-2920.14254. View

11.

Tossi V, Amenta M, Lamattina L, Cassia R . Nitric oxide enhances plant ultraviolet-B protection up-regulating gene expression of the phenylpropanoid biosynthetic pathway. Plant Cell Environ. 2011; 34(6):909-921. DOI: 10.1111/j.1365-3040.2011.02289.x. View

12.

Millar A, Whelan J, Soole K, Day D . Organization and regulation of mitochondrial respiration in plants. Annu Rev Plant Biol. 2011; 62:79-104. DOI: 10.1146/annurev-arplant-042110-103857. View

13.

Pineros M, Magalhaes J, Alves V, Kochian L . The physiology and biophysics of an aluminum tolerance mechanism based on root citrate exudation in maize. Plant Physiol. 2002; 129(3):1194-206. PMC: 166513. DOI: 10.1104/pp.002295. View

14.

Li Z, Yang S, Long W, Yang G, Shen Z . Hydrogen sulphide may be a novel downstream signal molecule in nitric oxide-induced heat tolerance of maize (Zea mays L.) seedlings. Plant Cell Environ. 2013; 36(8):1564-72. DOI: 10.1111/pce.12092. View

15.

Napoli C, de Nigris F, Williams-Ignarro S, Pignalosa O, Sica V, Ignarro L . Nitric oxide and atherosclerosis: an update. Nitric Oxide. 2006; 15(4):265-79. DOI: 10.1016/j.niox.2006.03.011. View

16.

Marcos A, Ramos M, Marcos J, Carmona L, Strauss J, Canovas D . Nitric oxide synthesis by nitrate reductase is regulated during development in Aspergillus. Mol Microbiol. 2015; 99(1):15-33. PMC: 4982101. DOI: 10.1111/mmi.13211. View

17.

Shi S, Wang G, Wang Y, Zhang L, Zhang L . Protective effect of nitric oxide against oxidative stress under ultraviolet-B radiation. Nitric Oxide. 2005; 13(1):1-9. DOI: 10.1016/j.niox.2005.04.006. View

18.

Costa J, Mota E, Cambursano M, Lauxmann M, de Oliveira L, Silva Lima M . Stress-induced co-expression of two alternative oxidase (VuAox1 and 2b) genes in Vigna unguiculata. J Plant Physiol. 2009; 167(7):561-70. DOI: 10.1016/j.jplph.2009.11.001. View

19.

Wang L, Wu X, Gao W, Zhao M, Zhang J, Huang C . Differential Expression Patterns of Pleurotus ostreatus Catalase Genes during Developmental Stages and under Heat Stress. Genes (Basel). 2017; 8(11). PMC: 5704248. DOI: 10.3390/genes8110335. View

20.

Saha B, Borovskii G, Panda S . Alternative oxidase and plant stress tolerance. Plant Signal Behav. 2016; 11(12):e1256530. PMC: 5225930. DOI: 10.1080/15592324.2016.1256530. View