Comparative Transcriptome Analysis of in Response to the Entomopathogenic Fungi and

Overview

Journal Insects

Specialty Biology

Date 2019 Dec 22

PMID 31861642

Citations 6

Authors

Zhongchen Rao

Li Cao

Hua Wu

Xuehong Qiu

Guiqing Liu

Richou Han

Affiliations

Soon will be listed here.

Abstract

is a medicinal and economically important Lepidopteran insect species. The larvae infected by survive no more than four days, while those infected by can survive for several months before mummification. This provides a valuable comparative system to study interactions between an insect host and different pathogenic fungi. By using the genome, a time-course transcriptome analysis of the whole larvae without guts was performed to explore the larvae response to and . A total of 3106 differentially expressed genes in five clusters were identified. The genes involved in coagulation and multiple metabolisms were both suppressed after or infection, whereas those related to environmental information responses, cell processes, biotic stimulus, and immunity (such as ()) were elevated. The rapid death of after infection might be caused by osmotic imbalance, immunocompromise (such as and ), and nervous system dysfunction (glutamatergic synapse). Up-regulation of the genes related to cuticle structure, nervous system (such as neurotrophin signal pathway and dopaminergic synapse) and immune effectors (such as () and ()) in , may contribute to the co-existence of and . This study provides a global view and potential key genes of the interaction between and two fungal entomopathogens.

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References

Mulinari S, Hacker U, Castillejo-Lopez C . Expression and regulation of Spätzle-processing enzyme in Drosophila. FEBS Lett. 2006; 580(22):5406-10. DOI: 10.1016/j.febslet.2006.09.009. View

Li X, Liu Q, Li W, Li Q, Qian Z, Liu X . A breakthrough in the artificial cultivation of Chinese cordyceps on a large-scale and its impact on science, the economy, and industry. Crit Rev Biotechnol. 2018; 39(2):181-191. DOI: 10.1080/07388551.2018.1531820. View

Xie C, Mao X, Huang J, Ding Y, Wu J, Dong S . KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases. Nucleic Acids Res. 2011; 39(Web Server issue):W316-22. PMC: 3125809. DOI: 10.1093/nar/gkr483. View

Wang X, Yao Y . Host insect species of Ophiocordyceps sinensis: a review. Zookeys. 2011; (127):43-59. PMC: 3175130. DOI: 10.3897/zookeys.127.802. View

Wichadakul D, Kobmoo N, Ingsriswang S, Tangphatsornruang S, Chantasingh D, Luangsa-Ard J . Insights from the genome of Ophiocordyceps polyrhachis-furcata to pathogenicity and host specificity in insect fungi. BMC Genomics. 2015; 16:881. PMC: 4625970. DOI: 10.1186/s12864-015-2101-4. View

Meng Q, Yu H, Zhang H, Zhu W, Wang M, Zhang J . Transcriptomic insight into the immune defenses in the ghost moth, Hepialus xiaojinensis, during an Ophiocordyceps sinensis fungal infection. Insect Biochem Mol Biol. 2015; 64:1-15. DOI: 10.1016/j.ibmb.2015.06.014. View

Morgan J . p Value fetishism and use of the Bonferroni adjustment. Evid Based Ment Health. 2007; 10(2):34-5. DOI: 10.1136/ebmh.10.2.34. View

Ogata H, Goto S, Sato K, Fujibuchi W, Bono H, Kanehisa M . KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 1998; 27(1):29-34. PMC: 148090. DOI: 10.1093/nar/27.1.29. View

Lo H, Hsieh C, Lin F, Hsu T . A Systematic Review of the Mysterious Caterpillar Fungus Ophiocordyceps sinensis in Dong-ChongXiaCao ( Dōng Chóng Xià Cǎo) and Related Bioactive Ingredients. J Tradit Complement Med. 2014; 3(1):16-32. PMC: 3924981. DOI: 10.4103/2225-4110.106538. View

10.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

11.

Bogus M, Kedra E, Bania J, Szczepanik M, Czygier M, Jablonski P . Different defense strategies of Dendrolimus pini, Galleria mellonella, and Calliphora vicina against fungal infection. J Insect Physiol. 2007; 53(9):909-22. DOI: 10.1016/j.jinsphys.2007.02.016. View

12.

Valanne S, Wang J, Ramet M . The Drosophila Toll signaling pathway. J Immunol. 2011; 186(2):649-56. DOI: 10.4049/jimmunol.1002302. View

13.

Zhong X, Gu L, Li S, Kan X, Zhang G, Liu X . Transcriptome analysis of Ophiocordyceps sinensis before and after infection of Thitarodes larvae. Fungal Biol. 2016; 120(6-7):819-26. DOI: 10.1016/j.funbio.2016.02.003. View

14.

Gottar M, Gobert V, Matskevich A, Reichhart J, Wang C, Butt T . Dual detection of fungal infections in Drosophila via recognition of glucans and sensing of virulence factors. Cell. 2006; 127(7):1425-37. PMC: 1865096. DOI: 10.1016/j.cell.2006.10.046. View

15.

Sung G, Hywel-Jones N, Sung J, Luangsa-Ard J, Shrestha B, Spatafora J . Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol. 2008; 57:5-59. PMC: 2104736. DOI: 10.3114/sim.2007.57.01. View

16.

Yue K, Ye M, Zhou Z, Sun W, Lin X . The genus Cordyceps: a chemical and pharmacological review. J Pharm Pharmacol. 2013; 65(4):474-93. DOI: 10.1111/j.2042-7158.2012.01601.x. View

17.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

18.

Lemaitre B, Hoffmann J . The host defense of Drosophila melanogaster. Annu Rev Immunol. 2007; 25:697-743. DOI: 10.1146/annurev.immunol.25.022106.141615. View

19.

de Hoon M, Imoto S, Nolan J, Miyano S . Open source clustering software. Bioinformatics. 2004; 20(9):1453-4. DOI: 10.1093/bioinformatics/bth078. View

20.

Jiang H, Kanost M . The clip-domain family of serine proteinases in arthropods. Insect Biochem Mol Biol. 2000; 30(2):95-105. DOI: 10.1016/s0965-1748(99)00113-7. View