Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2019 Dec 22

PMID 31861330

Citations 2

Authors

Shiwani Mandhania

Ajay Pal

Vinod Saharan

Affiliations

Soon will be listed here.

Abstract

Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid-acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious ultra-performance liquid chromatographic-photodiode array (UPLC-PDA) method for qualitative and quantitative analysis of 28 phenolic compounds comprising of flavonoids, phenolic acids, aldehydes and alcohols. The method is able to separate phenolic compounds in just 17 minutes with the separation of isobaric species such as 3,4 dihydroxybenzoic acid and 3,5 dihydroxy benzoic acid; quercetin and taxifolin. Linear curves concentrations ranged from 6-18 µg/mL (3,5 dihydroxy benzoic acid), 4-12 µg/mL (catechin and salicylic acid) and 2-6 µg/mL for rest of the compounds and correlation coefficients were >0.994. The limit of detection (LOD) varied from 0.04-0.45 µg/mL. Cotton root samples were used to assess the method in terms of recovery efficiency (85-120%), precision (0.12-4.09%) and intermediate precision (0.32-4.0%).Phenolics and flavonoidsin root samples of healthy and diseased plants as well as leaf samples of healthy plants were successfully quantified using this novel method without an expensive Mass Spectrometer.

Citing Articles

Morpho-physiological and biochemical responses of cotton ( L.) genotypes upon sucking insect-pest infestations.

Singh V, Mandhania S, Pal A, Kaur T, Banakar P, Sankaranarayanan K Physiol Mol Biol Plants. 2022; 28(11-12):2023-2039.

PMID: 36573153 PMC: 9789232. DOI: 10.1007/s12298-022-01253-w.

Optimization through a Box-Behnken Experimental Design of the Microwave-Assisted Extraction of the Psychoactive Compounds in Hallucinogenic Fungi ().

Polo-Castellano C, Alvarez J, Palma M, Barbero G, Ayuso J, Ferreiro-Gonzalez M J Fungi (Basel). 2022; 8(6).

PMID: 35736081 PMC: 9225378. DOI: 10.3390/jof8060598.

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