» Articles » PMID: 31851619

CircRNA_100367 Regulated the Radiation Sensitivity of Esophageal Squamous Cell Carcinomas Through MiR-217/Wnt3 Pathway

Overview
Specialty Geriatrics
Date 2019 Dec 19
PMID 31851619
Citations 80
Authors
Affiliations
Soon will be listed here.
Abstract

Background: Circular RNAs (circRNAs) play important roles in regulating the radioresistance of esophageal squamous cell carcinoma (ESCC). This study aimed to determine the role of hsa_circRNA_100367 in regulating radioresistance of ESCC.

Results: Higher expression and potency of endothelial to mesenchymal transformation (EMT) was found in radioresistant ESCC cells (KYSE-150R) than in ESCC cells (KYSE-150). Silencing circRNA_100367 inhibited the proliferation and migration of KYSE-150R cells, and decreased the expression of β-catenin (an important molecule in Wnt pathway) in KYSE-150R cells. Additionally, circRNA_100367 bound to miR-217, and miR-217 targeted Wnt3. Low Wnt3 expression was associated with the short survival time in patients with ESCC and Wnt3 knockdown inhibited the proliferation and migration of KYSE-150R cells. CircRNA_100367 enhanced the radioresistance of KYSE-150R cells through miR-217/Wnt3 pathway. , circRNA_100367 silence reduced the growth of KYSE-150R cells under radiation.

Conclusion: Our results revealed that circRNA_100367 attenuated radioresistance of ESCC through miR-217/Wnt3 pathway.

Methods: CircRNAs related with the radioresistance of ESCC were analyzed by hierarchical cluster analysis. The relationship between circRNA_100367 and miR-217, Wnt3 was detected by RNA immunoprecipitation (RIP), RNA pull-down and luciferase reporte assays. The proliferation and migration ESCC cells were detected by MTT, Transwell and colony formation assays.

Citing Articles

circ-TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy-Mediated Radiosensitivity Through miR-145-5p/SIRT1 Axis.

Liu Y, Lan S, Duan Z Thorac Cancer. 2024; 16(1):e15494.

PMID: 39621506 PMC: 11729993. DOI: 10.1111/1759-7714.15494.


Circ_0096710 facilitates tumor growth via controlling ADAM10 expression in esophageal squamous cell carcinoma.

Dong C, Li Z Thorac Cancer. 2024; 16(1):e15483.

PMID: 39620490 PMC: 11729450. DOI: 10.1111/1759-7714.15483.


Radioresistance and brain metastases: a review of the literature and applied perspective.

Youssef A, Sahgal A, Das S Front Oncol. 2024; 14:1477448.

PMID: 39540151 PMC: 11557554. DOI: 10.3389/fonc.2024.1477448.


CircFOXO3 upregulation mediates the radioresistance of glioblastoma by affecting cellular metabolome.

Xu H, Xing J, Cheng L, Wang Z, Zhao L, Ren L Front Pharmacol. 2024; 15:1479480.

PMID: 39449966 PMC: 11499195. DOI: 10.3389/fphar.2024.1479480.


Emerging roles of CircRNA-miRNA networks in cancer development and therapeutic response.

Hashemi M, Khosroshahi E, Daneii P, Hassanpoor A, Eslami M, Koohpar Z Noncoding RNA Res. 2024; 10:98-115.

PMID: 39351450 PMC: 11440256. DOI: 10.1016/j.ncrna.2024.09.006.


References
1.
Luo J, Wang W, Tang Y, Zhou D, Gao Y, Zhang Q . mRNA and methylation profiling of radioresistant esophageal cancer cells: the involvement of Sall2 in acquired aggressive phenotypes. J Cancer. 2017; 8(4):646-656. PMC: 5370508. DOI: 10.7150/jca.15652. View

2.
Zhang Y, Liu H, Li W, Yu J, Li J, Shen Z . CircRNA_100269 is downregulated in gastric cancer and suppresses tumor cell growth by targeting miR-630. Aging (Albany NY). 2017; 9(6):1585-1594. PMC: 5509457. DOI: 10.18632/aging.101254. View

3.
Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M . Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2014; 136(5):E359-86. DOI: 10.1002/ijc.29210. View

4.
Zhu H, Song H, Chen G, Yang X, Liu J, Ge Y . eEF2K promotes progression and radioresistance of esophageal squamous cell carcinoma. Radiother Oncol. 2017; 124(3):439-447. DOI: 10.1016/j.radonc.2017.04.001. View

5.
Xu L, Feng X, Hao X, Wang P, Zhang Y, Zheng X . CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth. J Exp Clin Cancer Res. 2019; 38(1):98. PMC: 6385474. DOI: 10.1186/s13046-019-1041-2. View