Phospholipid Analysis of Human Eosinophils: High Levels of Alkylacylglycerophosphocholine (PAF Precursor)

Overview

Journal Lipids

Specialty Biochemistry

Date 1988 Aug 1

PMID 3185116

Citations 11

Authors

Y Masuzawa

T Sugiura

K WAKU

H Saito

Y Yui

H Tomioka

Affiliations

Soon will be listed here.

Abstract

The phospholipid composition and fatty acid profiles of human eosinophils were studied. Extremely high levels of ether phospholipids were found in this type of cell, such as alkylacylglycerophosphocholine (GPC) and alkenylacylglycerophosphoethanolamine (GPE); these two ether phospholipids accounted for about three-fourths of the choline and ethanolamine glycerophospholipids (CGP and EGP), respectively. Fatty acid analyses revealed that very large portions of arachidonic acid (20:4) were esterified to alkylacyl-GPC (92.0% in CGP) and alkenylacyl-GPE (86.6% in EGP), respectively. While high amounts of alkylacyl-GPC and the abundance of 20:4 in this ether phospholipid have been observed in other types of blood cells of various animals, these percentages for human eosinophils are the highest among such cells. These results suggest that alkyl and alkenyl ether phospholipids play essential roles in human eosinophils in various physiological and pathological conditions.

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References

Albert D, Snyder F . Release of arachidonic acid from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine, a precursor of platelet-activating factor, in rat alveolar macrophages. Biochim Biophys Acta. 1984; 796(1):92-101. DOI: 10.1016/0005-2760(84)90242-x. View

Lee T, Lenihan D, Malone B, Roddy L, Wasserman S . Increased biosynthesis of platelet-activating factor in activated human eosinophils. J Biol Chem. 1984; 259(9):5526-30. View

Elsas P, Lee T, Lenzi H, Dessein A . Monocytes activate eosinophils for enhanced helminthotoxicity and increased generation of leukotriene C4. Ann Inst Pasteur Immunol. 1987; 138(1):97-116. DOI: 10.1016/s0769-2625(87)80099-5. View

Albert D, Snyder F . Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine by rat alveolar macrophages. Phospholipase A2 and acetyltransferase activities during phagocytosis and.... J Biol Chem. 1983; 258(1):97-102. View

Bruynzeel P, Kok P, Hamelink M, Kijne A, Verhagen J . Exclusive leukotriene C4 synthesis by purified human eosinophils induced by opsonized zymosan. FEBS Lett. 1985; 189(2):350-4. DOI: 10.1016/0014-5793(85)81054-1. View

Horn B, Robin E, Theodore J, Van Kessel A . Total eosinophil counts in the management of bronchial asthma. N Engl J Med. 1975; 292(22):1152-5. DOI: 10.1056/NEJM197505292922204. View

Wardlaw A, Moqbel R, Cromwell O, Kay A . Platelet-activating factor. A potent chemotactic and chemokinetic factor for human eosinophils. J Clin Invest. 1986; 78(6):1701-6. PMC: 423945. DOI: 10.1172/JCI112765. View

Mahmoud A, Warren K, Peters P . A role for the eosinophil in acquired resistance to Schistosoma mansoni infection as determined by antieosinophil serum. J Exp Med. 1975; 142(4):805-13. PMC: 2189955. DOI: 10.1084/jem.142.4.805. View

Swendsen C, Ellis J, Chilton 3rd F, OFlaherty J, Wykle R . 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine: a novel source of arachidonic acid in neutrophils stimulated by the calcium ionophore A23187. Biochem Biophys Res Commun. 1983; 113(1):72-9. DOI: 10.1016/0006-291x(83)90433-3. View

10.

Rouser G, Siakotos A, Fleischer S . Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots. Lipids. 1966; 1(1):85-6. DOI: 10.1007/BF02668129. View

11.

Taniguchi N, Mita H, Saito H, Yui Y, Kajita T, Shida T . Increased generation of leukotriene C4 from eosinophils in asthmatic patients. Allergy. 1985; 40(8):571-3. DOI: 10.1111/j.1398-9995.1985.tb00884.x. View

12.

Franklin W . Treatment of severe asthma. N Engl J Med. 1974; 290(26):1469-72. DOI: 10.1056/NEJM197406272902606. View

13.

GARTNER I . Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll). Immunology. 1980; 40(1):133-6. PMC: 1458478. View

14.

Vadas M, David J, Butterworth A, Pisani N, Siongok T . A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. J Immunol. 1979; 122(4):1228-36. View

15.

Billah M, Bryant R, Siegel M . Lipoxygenase products of arachidonic acid modulate biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by human neutrophils via phospholipase A2. J Biol Chem. 1985; 260(11):6899-906. View

16.

Nakagawa Y, Kurihara K, Sugiura T, WAKU K . Relative degradation of different arachidonoyl molecular species of choline glycerophospholipids in opsonized zymosan-stimulated rabbit alveolar macrophages. Biochim Biophys Acta. 1986; 876(3):601-10. View

17.

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

18.

Gupta S, Frenkel R, Rosenstein M, GRIECO M . Lymphocyte subpopulations, serum IgE and total eosinophil counts in patients with bronchial asthma. Clin Exp Immunol. 1975; 22(3):438-45. PMC: 1538426. View

19.

Butterworth A, David J . Eosinophil function. N Engl J Med. 1981; 304(3):154-6. DOI: 10.1056/NEJM198101153040305. View

20.

Frigas E, Loegering D, Gleich G . Cytotoxic effects of the guinea pig eosinophil major basic protein on tracheal epithelium. Lab Invest. 1980; 42(1):35-43. View