5,6-Epoxyphytosterols and 5,6-Epoxycholesterol Increase Oxidative Stress in Rats on Low-Cholesterol Diet

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2019 Dec 13

PMID 31827670

Citations 2

Authors

Tomasz Wielkoszynski

Jolanta Zalejska-Fiolka

Joanna K Strzelczyk

Aleksander J Owczarek

Armand Cholewka

Aneta Krawczyk

Agata Stanek

Affiliations

Soon will be listed here.

Abstract

Objective: Cholesterol oxidation products have an established proatherogenic and cytotoxic effect. An increased exposure to these substances may be associated with the development of atherosclerosis and cancers. Relatively little, though, is known about the effect of phytosterol oxidation products, although phytosterols are present in commonly available and industrial food products. Thus, the aim of the research was to assess the effect of 5,6-epoxyphytosterols, which are important phytosterol oxidation products, on redox state in rats.

Material And Methods: The animals were divided into 3 groups and exposed to nutritional sterols by receiving feed containing 5,6-epoxyphytosterols (ES group) and 5,6-epoxycholesterol (Ech group) or sterol-free feed (C group). The levels of malondialdehyde (MDA), conjugated dienes (CD), and ferric reducing antioxidant potential (FRAP) were assayed in the plasma; anti-7-ketocholesterol antibodies and activity of paraoxonase-1 (PON1) were determined in serum, whereas the activity of catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), S-glutathione transferase (GST), and superoxide dismutase (SOD) were assayed in RBCs.

Results: During the experiment, the levels of lipid peroxidation products increased, such as CD and anti-7-ketocholesterol antibodies. At the same time, the plasma levels of FRAP and serum activity of PON1 decreased alongside the reduced activity of GPx, GR, and SOD in RBCs. There was no effect of the studied compounds on the plasma MDA levels or on the activity of CAT and GST in RBCs.

Conclusions: Both 5,6-epoxyphytosterols and 5,6-epoxycholesterols similarly dysregulate the state in experimental animal model and may significantly impact atherogenesis.

Citing Articles

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PMID: 36593033 PMC: 9843796. DOI: 10.21873/invivo.13069.

5,6-Epoxyphytosterols and 5,6-Epoxycholesterol Increase Nitrosative Stress and Inflammatory Cytokine Production in Rats on Low-Cholesterol Diet.

Wielkoszynski T, Zalejska-Fiolka J, Strzelczyk J, Owczarek A, Cholewka A, Kokoszczyk K Oxid Med Cell Longev. 2020; 2020:4751803.

PMID: 32587660 PMC: 7298340. DOI: 10.1155/2020/4751803.

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