Cathelicidin Contributes to the Restriction of in Human Host Macrophages

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2019 Dec 12

PMID 31824492

Citations 10

Authors

Peter Crauwels

Elena Bank

Bianca Walber

Ulf Alexander Wenzel

Birgitta Agerberth

Menberework Chanyalew

Markos Abebe

Renate Konig

Uwe Ritter

Norbert Reiling

Ger van Zandbergen

Affiliations

Soon will be listed here.

Abstract

In cutaneous Leishmaniasis the parasitic control in human host macrophages is still poorly understood. We found an increased expression of the human cathelicidin in skin lesions of Ethiopian patients with cutaneous leishmaniasis. Vitamin D driven, Cathelicidin-type antimicrobial peptides (CAMP) play an important role in the elimination of invading microorganisms. Recombinant cathelicidin was able to induce cell-death characteristics in in a dose dependent manner. Using human primary macrophages, we demonstrated pro-inflammatory macrophages (hMDM1) to express a higher level of human cathelicidin, both on gene and protein level, compared to anti-inflammatory macrophages (hMDM2). Activating the CAMP pathway using Vitamin D in hMDM1 resulted in a cathelicidin-mediated- restriction. Finally, a reduction of cathelicidin in hMDM1, using a RNA interference (RNAi) approach, increased parasite survival. In all, these data show the human cathelicidin to contribute to the innate immune response against Leishmaniasis in a human primary cell model.

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