A Novel Detection of Using Multiple Cross Displacement Amplification Linked with Gold Nanoparticle Lateral Flow Biosensor

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2019 Dec 12

PMID 31824177

Citations 4

Authors

Xu Chen

Kai Ma

Xu Yi

Ziyu Xiao

Lijuan Xiong

Yu Wang

ShiJun Li

Affiliations

Soon will be listed here.

Abstract

Background: , an opportunistic bacterial pathogen, is one of the most frequently isolated bacterial species and cause of serious nosocomial infections in recent decades. A reliable and rapid assay for detection is significant for the diagnosis and follow-up treatment.

Methods: A novel assay method, named multiple cross displacement amplification linked with nanoparticle-based lateral flow biosensor (MCDA-LFB), was applied for detecting strains. A set of special 10 primers was designed according to -specific gene . The MCDA amplification conditions, including the target DNA concentration, reaction temperature and time, were optimized. The sensitivity and specificity of MCDA method were tested in the current study, and then, the MCDA-LFB technology was applied to detect the strain from clinical samples.

Results: The specific primers were valid for the establishment of MCDA-LFB technology forthe detection of based on the gene. The MCDA amplification condition was optimized at 62°C for 35 min. The MCDA products were directly sensed and displayed with a biosensor. The full process, comprising genomic DNA template preparation (approximately 30 mins), amplification of MCDA (35 mins), and the product identification (approximately 2 mins), could be achieved in 70 mins. The MCDA technique could detect as little as 10 fg per reaction system of pure genomic DNA. The specificity of MCDA-LFB method is 100%, with no cross-reactions to non- strains.

Conclusion: The MCDA-LFB technique established in the present study is a reliable, simple, rapid, sensitive and specific method to assay and can be applied for the detection of clinical samples.

Citing Articles

Advances in the Application of Nanomaterials as Treatments for Bacterial Infectious Diseases.

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Rapid and Visual Differentiation of From the Complex Using Multiplex Loop-Mediated Isothermal Amplification Coupled With a Nanoparticle-Based Lateral Flow Biosensor.

Yang X, Huang J, Chen X, Xiao Z, Wang X, Chen Y Front Microbiol. 2021; 12:708658.

PMID: 34408738 PMC: 8365424. DOI: 10.3389/fmicb.2021.708658.

Highly specific and sensitive detection of the Mycobacterium tuberculosis complex using multiplex loop-mediated isothermal amplification combined with a nanoparticle-based lateral flow biosensor.

Chen X, Huang J, Xiao Z, Yang X, Chen Y, Zheng W Braz J Microbiol. 2021; 52(3):1315-1325.

PMID: 34176103 PMC: 8324766. DOI: 10.1007/s42770-021-00520-4.

Multiple Cross Displacement Amplification Linked with Nanoparticles-Based Lateral Flow Biosensor in Screening of Hepatitis B Virus in Clinical Application.

Chen X, Zhou Q, Dong S, Wang S, Liu R, Wu X Infect Drug Resist. 2021; 14:1219-1229.

PMID: 33790592 PMC: 8007573. DOI: 10.2147/IDR.S297645.

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