Prevalence and Phylogenetic Relationship Among Methicillin- and Vancomycin-resistant Isolated from Hospital's Dairy Food, Food Handlers, and Patients

Overview

Journal J Adv Vet Anim Res

Specialty Veterinary Medicine

Date 2019 Dec 11

PMID 31819873

Citations 7

Authors

Mona Ahmed El-Zamkan

Asmaa Gaber Mubarak

Alsagher Omran Ali

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of the present work was to investigate the mutual role that may be played by the served dairy food and food handlers in the transmission of methicillin- and vancomycin-resistant and coagulase-negative to patients who were hospitalized in Qena City, Egypt.

Materials And Methods: A total of 210 samples including 90 dairy food samples which offered to the patients in the hospital, 60 nasal and hand swabs from food handlers working in the hospital, and 60 nasal and diarrheal swabs from patients suffering from diarrhea were investigated for the presence of coagulase-positive and coagulase-negative , then isolates were screened for methicillin and vancomycin resistance phenotypically and genotypically. gene sequencing was employed to construct the neighbor-joining tree.

Results: Unlike food samples, both coagulase-positive and coagulase-negative occurred in human samples. Methicillin- and vancomycin-resistant coagulase-negative could be detected in 41.7% & 20.8%, 68% & 31.9%, and 81.3% & 55.2% of isolates obtained from dairy food, food handlers, and patients' samples, respectively. Whereas 81% & 64.3%, and 75.4% & 38.6% of coagulase-positive obtained from food handlers and patients' samples exhibited resistance to methicillin and vancomycin, respectively. Phenotypic resistance was confirmed molecularly through detection of and genes.

Conclusion: A significant role can be played by food and food handlers in the transmission of methicillin- and vancomycin-resistant to patients, which has been proved in this study through the close phylogenetic relation between isolated from food, food handlers, and patients' diarrheal samples.

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