Fluorescent Single-Walled Carbon Nanotubes for Protein Detection

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2019 Dec 11

PMID 31817932

Citations 19

Authors

Adi Hendler-Neumark

Gili Bisker

Affiliations

Soon will be listed here.

Abstract

Nanosensors have a central role in recent approaches to molecular recognition in applications like imaging, drug delivery systems, and phototherapy. Fluorescent nanoparticles are particularly attractive for such tasks owing to their emission signal that can serve as optical reporter for location or environmental properties. Single-walled carbon nanotubes (SWCNTs) fluoresce in the near-infrared part of the spectrum, where biological samples are relatively transparent, and they do not photobleach or blink. These unique optical properties and their biocompatibility make SWCNTs attractive for a variety of biomedical applications. Here, we review recent advancements in protein recognition using SWCNTs functionalized with either natural recognition moieties or synthetic heteropolymers. We emphasize the benefits of the versatile applicability of the SWCNT sensors in different systems ranging from single-molecule level to in-vivo sensing in whole animal models. Finally, we discuss challenges, opportunities, and future perspectives.

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References

Bisker G, Bakh N, Lee M, Ahn J, Park M, OConnell E . Insulin Detection Using a Corona Phase Molecular Recognition Site on Single-Walled Carbon Nanotubes. ACS Sens. 2018; 3(2):367-377. DOI: 10.1021/acssensors.7b00788. View

Efros A, Nesbitt D . Origin and control of blinking in quantum dots. Nat Nanotechnol. 2016; 11(8):661-71. DOI: 10.1038/nnano.2016.140. View

Bisker G, Dong J, Park H, Iverson N, Ahn J, Nelson J . Protein-targeted corona phase molecular recognition. Nat Commun. 2016; 7:10241. PMC: 4729864. DOI: 10.1038/ncomms10241. View

Beyene A, Delevich K, Del Bonis-ODonnell J, Piekarski D, Lin W, Thomas A . Imaging striatal dopamine release using a nongenetically encoded near infrared fluorescent catecholamine nanosensor. Sci Adv. 2019; 5(7):eaaw3108. PMC: 6620097. DOI: 10.1126/sciadv.aaw3108. View

Michalet X, Pinaud F, Bentolila L, Tsay J, Doose S, Li J . Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709):538-44. PMC: 1201471. DOI: 10.1126/science.1104274. View

Gravely M, Safaee M, Roxbury D . Biomolecular Functionalization of a Nanomaterial To Control Stability and Retention within Live Cells. Nano Lett. 2019; 19(9):6203-6212. PMC: 7199458. DOI: 10.1021/acs.nanolett.9b02267. View

Dineshkumar B, Krishnakumar K, Bhatt A, Paul D, Cherian J, John A . Single-walled and multi-walled carbon nanotubes based drug delivery system: Cancer therapy: A review. Indian J Cancer. 2016; 52(3):262-4. DOI: 10.4103/0019-509X.176720. View

Hernandez-Rivera M, Zaibaq N, Wilson L . Toward carbon nanotube-based imaging agents for the clinic. Biomaterials. 2016; 101:229-40. DOI: 10.1016/j.biomaterials.2016.05.045. View

Wang F, Dukovic G, Brus L, Heinz T . The optical resonances in carbon nanotubes arise from excitons. Science. 2005; 308(5723):838-41. DOI: 10.1126/science.1110265. View

10.

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

11.

Cherukuri P, Bachilo S, Litovsky S, Weisman R . Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells. J Am Chem Soc. 2004; 126(48):15638-9. DOI: 10.1021/ja0466311. View

12.

Alvarez M, Aizenberg J, Analoui M, Andrews A, Bisker G, Boyden E . Emerging Trends in Micro- and Nanoscale Technologies in Medicine: From Basic Discoveries to Translation. ACS Nano. 2017; 11(6):5195-5214. DOI: 10.1021/acsnano.7b01493. View

13.

Shimomura O, Johnson F, Saiga Y . Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol. 1962; 59:223-39. DOI: 10.1002/jcp.1030590302. View

14.

Lee K, Lee J, Ahn B . Design of Refolding DNA Aptamer on Single-Walled Carbon Nanotubes for Enhanced Optical Detection of Target Proteins. Anal Chem. 2019; 91(20):12704-12712. DOI: 10.1021/acs.analchem.9b02177. View

15.

Landry M, Vukovic L, Kruss S, Bisker G, Landry A, Islam S . Comparative Dynamics and Sequence Dependence of DNA and RNA Binding to Single Walled Carbon Nanotubes. J Phys Chem C Nanomater Interfaces. 2015; 119(18):10048-10058. PMC: 4440682. DOI: 10.1021/jp511448e. View

16.

Beyene A, Alizadehmojarad A, Dorlhiac G, Goh N, Streets A, Kral P . Ultralarge Modulation of Fluorescence by Neuromodulators in Carbon Nanotubes Functionalized with Self-Assembled Oligonucleotide Rings. Nano Lett. 2018; 18(11):6995-7003. PMC: 6771428. DOI: 10.1021/acs.nanolett.8b02937. View

17.

McHugh K, Jing L, Behrens A, Jayawardena S, Tang W, Gao M . Biocompatible Semiconductor Quantum Dots as Cancer Imaging Agents. Adv Mater. 2018; 30(18):e1706356. DOI: 10.1002/adma.201706356. View

18.

Beyene A, Demirer G, Landry M . Nanoparticle-Templated Molecular Recognition Platforms for Detection of Biological Analytes. Curr Protoc Chem Biol. 2016; 8(3):197-223. PMC: 10539024. DOI: 10.1002/cpch.10. View

19.

Alivisatos A, Gu W, Larabell C . Quantum dots as cellular probes. Annu Rev Biomed Eng. 2005; 7:55-76. DOI: 10.1146/annurev.bioeng.7.060804.100432. View

20.

Landry M, Kruss S, Nelson J, Bisker G, Iverson N, Reuel N . Experimental tools to study molecular recognition within the nanoparticle corona. Sensors (Basel). 2014; 14(9):16196-211. PMC: 4208170. DOI: 10.3390/s140916196. View