Efficacy Of Line Probe Assay In Detection Of Drug-Resistant Pulmonary Tuberculosis In Comparison With GeneXpert And Phenotypic Methods In Iran And Genetic Analysis Of Isolates By MIRU-VNTR

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2019 Dec 10

PMID 31814746

Citations 6

Authors

Hossein Kazemian

Jalil Kardan-Yamchi

Abbas Bahador

Shadi Khonsari

Mahshid Nasehi

Gholamreza Hamzehloo

Farzam Vaziri

Mohammad Reza Salehi

Mohammad Mehdi Feizabadi

Affiliations

Soon will be listed here.

Abstract

Background: Successful treatment of tuberculosis depends on early diagnosis and use of appropriate drug susceptibility testing in a timely manner. In the present study, LPA efficacy was assayed in detection and drug susceptibility testing of pulmonary tuberculosis in comparison to available methods in Iran and phylogenetic analyses of isolated cases carried out by MIRU-VNTR.

Methods: This study was conducted at the Tehran Regional Reference Laboratory for Tuberculosis. All sputum specimens were subjected to smear, culture, and drug susceptibility testing (DST), GeneXpert, and LPA. Finally, 15-locus-based MIRU-VNTR was used for molecular genotyping.

Results: From a total of 920 sputum specimens, 6.08% (n=56) were identified as MTBC by culture, 6.8% (n=63) by GeneXpert, and 6.5% (n=60) by LPA. Phenotype DST and LPA methods confirmed the resistance of 4 and 14 specimens to rifampin (RIF) and isoniazid (INH); two cases were considered as multidrug-resistant (MDR). Using GeneXpert, four cases were identified as RIF-resistant. Based on LPA results, and mutations were detected in 100% and 21.4% of INH-resistant cases, respectively. All 56 culture positive isolates were placed in 29 different clusters using MIRU-VNTR genotyping. Two MDR-TB, 2 RIF mono-resistant, and 12 INH mono-resistant cases were placed in different clusters.

Conclusion: LPA is an appropriate method for early detection and accurate diagnosis of TB and drug-resistant cases that makes it possible to distinguish INH mono-resistant cases from MDR cases in Iran.

Citing Articles

Epidemiology of first- and second-line drugs-resistant pulmonary tuberculosis in Iran: Systematic review and meta-analysis.

Abbasian S, Heidari H, Tadi D, Kardan-Yamchi J, Taji A, Darbandi A J Clin Tuberc Other Mycobact Dis. 2024; 35:100430.

PMID: 38560029 PMC: 10981085. DOI: 10.1016/j.jctube.2024.100430.

Comparative Performance of Line Probe Assay and GeneXpert in the Detection of Rifampicin Monoresistance in a TB-Endemic African Country.

Mchaki B, Mgaya F, Kunambi P, Hangombe B, Matee M, Munyeme M Antibiotics (Basel). 2022; 11(11).

PMID: 36358145 PMC: 9686643. DOI: 10.3390/antibiotics11111489.

The Value of GeneXpert MTB/RIF for Detection in Tuberculosis: A Bibliometrics-Based Analysis and Review.

Li Z J Anal Methods Chem. 2022; 2022:2915018.

PMID: 36284547 PMC: 9588380. DOI: 10.1155/2022/2915018.

A Saudi Arabian Public Health Perspective of Tuberculosis.

Saati A, Khurram M, Faidah H, Haseeb A, Iriti M Int J Environ Res Public Health. 2021; 18(19).

PMID: 34639342 PMC: 8508237. DOI: 10.3390/ijerph181910042.

Magnitude of Phenotypic and MTBDRplus Line Probe Assay First-Line Anti-Tuberculosis Drug Resistance Among Tuberculosis Patients; Northwest Ethiopia.

Yigzaw W, Torrelles J, Wang S, Tessema B Infect Drug Resist. 2021; 14:497-505.

PMID: 33603414 PMC: 7882791. DOI: 10.2147/IDR.S292058.

References

Ninan M, Gowri M, Christopher D, Rupali P, Michael J . The diagnostic utility of line probe assays for multidrug-resistant tuberculosis. Pathog Glob Health. 2016; 110(4-5):194-9. PMC: 5072114. DOI: 10.1080/20477724.2016.1214350. View

Kadivar M, Ghaneh-Shirazi R, Khavandegaran F, Karimi M . Epidemiology of tuberculosis among Afghan immigrants in Fars province, southern Islamic Republic of Iran. East Mediterr Health J. 2007; 13(4):758-64. View

Boehme C, Nabeta P, Hillemann D, Nicol M, Shenai S, Krapp F . Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010; 363(11):1005-15. PMC: 2947799. DOI: 10.1056/NEJMoa0907847. View

Yamchi J, Haeili M, Feyisa S, Kazemian H, Hashemi Shahraki A, Zahednamazi F . Evaluation of efflux pump gene expression among drug susceptible and drug resistant strains of Mycobacterium tuberculosis from Iran. Infect Genet Evol. 2015; 36:23-26. DOI: 10.1016/j.meegid.2015.08.036. View

Kazemian H, Kardan-Yamchi J, Mosavari N, Feizabadi M . Molecular characterization of multidrug and extensive drug-resistant Mycobacterium tuberculosis isolates from Iran. Infez Med. 2019; 27(1):26-31. View

Gillespie S . Evolution of drug resistance in Mycobacterium tuberculosis: clinical and molecular perspective. Antimicrob Agents Chemother. 2002; 46(2):267-74. PMC: 127054. DOI: 10.1128/AAC.46.2.267-274.2002. View

Zignol M, Van Gemert W, Falzon D, Sismanidis C, Glaziou P, Floyd K . Surveillance of anti-tuberculosis drug resistance in the world: an updated analysis, 2007-2010. Bull World Health Organ. 2012; 90(2):111-119D. PMC: 3302554. DOI: 10.2471/BLT.11.092585. View

Mekonnen D, Admassu A, Mulu W, Amor A, Benito A, Gelaye W . Multidrug-resistant and heteroresistant Mycobacterium tuberculosis and associated gene mutations in Ethiopia. Int J Infect Dis. 2015; 39:34-8. DOI: 10.1016/j.ijid.2015.06.013. View

Small P, Hopewell P, Singh S, Paz A, Parsonnet J, Ruston D . The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods. N Engl J Med. 1994; 330(24):1703-9. DOI: 10.1056/NEJM199406163302402. View

10.

Van Helden P, Victor T, Warren R, van Helden E . Isolation of DNA from Mycobacterium tubercolosis. Methods Mol Med. 2011; 54:19-30. DOI: 10.1385/1-59259-147-7:019. View

11.

Barnard M, Gey van Pittius N, Van Helden P, Bosman M, Coetzee G, Warren R . The diagnostic performance of the GenoType MTBDRplus version 2 line probe assay is equivalent to that of the Xpert MTB/RIF assay. J Clin Microbiol. 2012; 50(11):3712-6. PMC: 3486209. DOI: 10.1128/JCM.01958-12. View

12.

Gandhi N, Brust J, Moodley P, Weissman D, Heo M, Ning Y . Minimal diversity of drug-resistant Mycobacterium tuberculosis strains, South Africa. Emerg Infect Dis. 2014; 20(3):426-33. PMC: 3944869. DOI: 10.3201/eid2003.131083. View

13.

Muller B, Streicher E, Hoek K, Tait M, Trollip A, Bosman M . inhA promoter mutations: a gateway to extensively drug-resistant tuberculosis in South Africa?. Int J Tuberc Lung Dis. 2011; 15(3):344-51. View

14.

Zamani S, Aflaki M, Fooladi A, Darban-Sarokhalil D, Bameri Z, Khazaee S . MIRU-VNTR analysis of the Mycobacterium tuberculosis isolates from three provinces of Iran. Scand J Infect Dis. 2012; 45(2):124-30. DOI: 10.3109/00365548.2012.717233. View

15.

Varghese B, Shoukri M, Memish Z, Abuljadayel N, Alhakeem R, AlRabiah F . Occurrence of diverse mutations in isoniazid- and rifampicin-resistant Mycobacterium tuberculosis isolates from autochthonous and immigrant populations of Saudi Arabia. Microb Drug Resist. 2014; 20(6):623-31. DOI: 10.1089/mdr.2014.0065. View

16.

Crudu V, Stratan E, Romancenco E, Allerheiligen V, Hillemann A, Moraru N . First evaluation of an improved assay for molecular genetic detection of tuberculosis as well as rifampin and isoniazid resistances. J Clin Microbiol. 2012; 50(4):1264-9. PMC: 3318528. DOI: 10.1128/JCM.05903-11. View

17.

Desikan P, Panwalkar N, Mirza S, Chaturvedi A, Ansari K, Varathe R . Line probe assay for detection of complex: An experience from Central India. Indian J Med Res. 2017; 145(1):70-73. PMC: 5460577. DOI: 10.4103/ijmr.IJMR_831_14. View

18.

Almeida Da Silva P, Palomino J . Molecular basis and mechanisms of drug resistance in Mycobacterium tuberculosis: classical and new drugs. J Antimicrob Chemother. 2011; 66(7):1417-30. DOI: 10.1093/jac/dkr173. View

19.

Shenoy V, Kumar A, Chawla K . Rapid detection of multidrug resistant tuberculosis in respiratory specimens at a tertiary care centre in south coastal Karnataka using Genotype MTBDR assay. Iran J Microbiol. 2019; 10(5):275-280. PMC: 6339998. View

20.

Nasiri M, Dabiri H, Darban-Sarokhalil D, Rezadehbashi M, Zamani S . Prevalence of drug-resistant tuberculosis in Iran: systematic review and meta-analysis. Am J Infect Control. 2014; 42(11):1212-8. DOI: 10.1016/j.ajic.2014.07.017. View