LncRNA EPIC1 Downregulation Mediates Hydrogen Peroxide-induced Neuronal Cell Injury

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2019 Dec 9

PMID 31812951

Citations 5

Authors

Jian Sun

Jinyu Zheng

Ya Li

Ming Yan

Ping Li

Lili Ma

Affiliations

Soon will be listed here.

Abstract

Excessive oxidative stress causes neuronal cell injury. Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a MYC-interacting LncRNA. Its expression and potential functions in hydrogen peroxide (HO)-stimulated neuronal cells are studied. In SH-SY5Y neuronal cells and primary human neuron cultures, HO downregulated Lnc-EPIC1 and key MYC targets , and ). Ectopic overexpression of Lnc-EPIC1 increased expression of MYC targets and significantly attenuated HO-induced neuronal cell death and apoptosis. Contrarily, Lnc-EPIC1 siRNA potentiated neuronal cell death by HO. MYC knockout by CRISPR/Cas9 method also facilitated HO-induced SH-SY5Y cell death. Significantly, MYC knockout abolished Lnc-EPIC1-induced actions in HO-stimulated neuronal cells. Together, these results suggest that Lnc-EPIC1 downregulation mediates HO-induced neuronal cell death.

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