Seventy-kilodalton Heat Shock Proteins and an Additional Component from Reticulocyte Lysate Stimulate Import of M13 Procoat Protein into Microsomes

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1988 Sep 1

PMID 3181144

Citations 53

Authors

R Zimmermann

M Sagstetter

M J Lewis

H R Pelham

Affiliations

Soon will be listed here.

Abstract

Processing of M13 procoat protein, synthesized in a bacterial cell-free extract, to transmembrane coat protein by dog pancreas microsomes is stimulated by a system which is present in rabbit reticulocytes and depends on nucleoside triphosphates. This system consists of (at least) two components which act synergistically: members of the 70-kd heat shock protein family and (at least) one additional component. This component depends on ATP (or GTP) for its action.

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References

Goodman J, Watts C, Wickner W . Membrane assembly: posttranslational insertion of M13 procoat protein into E. coli membranes and its proteolytic conversion to coat protein in vitro. Cell. 1981; 24(2):437-41. DOI: 10.1016/0092-8674(81)90334-2. View

Rothblatt J, Meyer D . Secretion in yeast: translocation and glycosylation of prepro-alpha-factor in vitro can occur via an ATP-dependent post-translational mechanism. EMBO J. 1986; 5(5):1031-6. PMC: 1166897. DOI: 10.1002/j.1460-2075.1986.tb04318.x. View

Stueber D, Ibrahimi I, Cutler D, Dobberstein B, Bujard H . A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences. EMBO J. 1984; 3(13):3143-8. PMC: 557830. DOI: 10.1002/j.1460-2075.1984.tb02271.x. View

Chen L, Tai P . ATP is essential for protein translocation into Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A. 1985; 82(13):4384-8. PMC: 390418. DOI: 10.1073/pnas.82.13.4384. View

Welch W, Feramisco J . Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides. Mol Cell Biol. 1985; 5(6):1229-37. PMC: 366850. DOI: 10.1128/mcb.5.6.1229-1237.1985. View

Mueckler M, Lodish H . The human glucose transporter can insert posttranslationally into microsomes. Cell. 1986; 44(4):629-37. DOI: 10.1016/0092-8674(86)90272-2. View

Lewis M, Pelham H . Involvement of ATP in the nuclear and nucleolar functions of the 70 kd heat shock protein. EMBO J. 1985; 4(12):3137-43. PMC: 554633. DOI: 10.1002/j.1460-2075.1985.tb04056.x. View

Perara E, Rothman R, Lingappa V . Uncoupling translocation from translation: implications for transport of proteins across membranes. Science. 1986; 232(4748):348-52. DOI: 10.1126/science.3961485. View

Hansen W, Garcia P, Walter P . In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor. Cell. 1986; 45(3):397-406. DOI: 10.1016/0092-8674(86)90325-9. View

10.

Waters M, Blobel G . Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis. J Cell Biol. 1986; 102(5):1543-50. PMC: 2114218. DOI: 10.1083/jcb.102.5.1543. View

11.

Geller B, Movva N, Wickner W . Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles. Proc Natl Acad Sci U S A. 1986; 83(12):4219-22. PMC: 323703. DOI: 10.1073/pnas.83.12.4219. View

12.

Rothman J, Schmid S . Enzymatic recycling of clathrin from coated vesicles. Cell. 1986; 46(1):5-9. DOI: 10.1016/0092-8674(86)90852-4. View

13.

SCHLESINGER M . Heat shock proteins: the search for functions. J Cell Biol. 1986; 103(2):321-5. PMC: 2113812. DOI: 10.1083/jcb.103.2.321. View

14.

Mueckler M, Lodish H . Post-translational insertion of a fragment of the glucose transporter into microsomes requires phosphoanhydride bond cleavage. Nature. 1986; 322(6079):549-52. DOI: 10.1038/322549a0. View

15.

Zimmermann R, Mollay C . Import of honeybee prepromelittin into the endoplasmic reticulum. Requirements for membrane insertion, processing, and sequestration. J Biol Chem. 1986; 261(27):12889-95. View

16.

Pelham H . Speculations on the functions of the major heat shock and glucose-regulated proteins. Cell. 1986; 46(7):959-61. DOI: 10.1016/0092-8674(86)90693-8. View

17.

Connolly T, Gilmore R . Formation of a functional ribosome-membrane junction during translocation requires the participation of a GTP-binding protein. J Cell Biol. 1986; 103(6 Pt 1):2253-61. PMC: 2114577. DOI: 10.1083/jcb.103.6.2253. View

18.

Pfanner N, Neupert W . Transport of F1-ATPase subunit beta into mitochondria depends on both a membrane potential and nucleoside triphosphates. FEBS Lett. 1986; 209(2):152-6. DOI: 10.1016/0014-5793(86)81101-2. View

19.

Waters M, Chirico W, Blobel G . Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor. J Cell Biol. 1986; 103(6 Pt 2):2629-36. PMC: 2114630. DOI: 10.1083/jcb.103.6.2629. View

20.

Chen W, DOUGLAS M . Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix. Cell. 1987; 49(5):651-8. DOI: 10.1016/0092-8674(87)90541-1. View