Endophytic Bacillus Velezensis Strain B-36 is a Potential Biocontrol Agent Against Lotus Rot Caused by Fusarium Oxysporum

Overview

Journal J Appl Microbiol

Publisher Oxford University Press

Date 2019 Dec 7

PMID 31808212

Citations 13

Authors

G F Wang

J F Meng

T Tian

X Q Xiao

B Zhang

Y N Xiao

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this study was to screen potential lotus plant endophytic bacterial isolate for effective inhibition against lotus rot causing fungal pathogen Fusarium oxysporum.

Methods And Results: In this study, endophytic bacteria were isolated from lotus tissues and tested for antagonistic activities against the pathogenic fungus F. oxysporum. Among the putative endophytic Bacillus strains identified, suspensions of the strain B-36 showed the highest inhibition rate against F. oxysporum growth. Pot assays indicated that B-36 was effective in controlling F. oxysporum-inducing lotus rot. However, the control efficiency varied with the inoculation method and concentration, where injection of 800 μl B-36 suspension per plant (2 × 10 CFU per ml) into stems showed the highest control efficiencies of 77·1 and 60·0% for pre-inoculation and post-inoculation. In addition, the colonizing population levels (CPLs) of B-36 on lotus also varied with the inoculation method and concentration, with the highest CPLs, that is, 3·05 and 2·83 log(CFU per gram), being observed on lotus leaves and stems respectively for stem injection of 200 μl per plant. Moreover B-36 showed no noticeable effects on lotus seed germination rate or seedling growth. Finally, B-36 was characterized as Bacillus velezensis based on its morphology, Gram-positive characteristics, as well as its 16S rDNA and gyrB sequences.

Conclusion: The isolate B-36 can be applied as a biocontrol agent against F. oxysporum-inducing lotus rot.

Significance Of Impact Of The Study: The soil-borne fungus F. oxysporum causes lotus rot and severe yield loss, and currently available control methods are very limited. Here we identify a new promising biocontrol agent against lotus rot caused by F. oxysporum.

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References

Ji S, Paul N, Deng J, Kim Y, Yun B, Yu S . Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases. Mycobiology. 2014; 41(4):234-42. PMC: 3905128. DOI: 10.5941/MYCO.2013.41.4.234. View

Etebarian H, Sholberg P, Eastwell K, Sayler R . Biological control of apple blue mold with Pseudomonas fluorescens. Can J Microbiol. 2005; 51(7):591-8. DOI: 10.1139/w05-039. View

Romano A, Vitullo D, Di Pietro A, Lima G, Lanzotti V . Antifungal lipopeptides from Bacillus amyloliquefaciens strain BO7. J Nat Prod. 2011; 74(2):145-51. DOI: 10.1021/np100408y. View

Kim Y, Kang H, Kwon K, Seo C, Lee H, Park Y . Antagonistic Activities of Novel Peptides from Bacillus amyloliquefaciens PT14 against Fusarium solani and Fusarium oxysporum. J Agric Food Chem. 2015; 63(48):10380-7. DOI: 10.1021/acs.jafc.5b04068. View

Sharma B, Gautam L, Adhikari D, Karki R . A Comprehensive Review on Chemical Profiling of Nelumbo Nucifera: Potential for Drug Development. Phytother Res. 2016; 31(1):3-26. DOI: 10.1002/ptr.5732. View

Yamamoto S, Shiraishi S, Suzuki S . Are cyclic lipopeptides produced by Bacillus amyloliquefaciens S13-3 responsible for the plant defence response in strawberry against Colletotrichum gloeosporioides?. Lett Appl Microbiol. 2014; 60(4):379-86. DOI: 10.1111/lam.12382. View

Mora I, Cabrefiga J, Montesinos E . Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant-Associated Bacillus against Phytopathogenic Bacteria. PLoS One. 2015; 10(5):e0127738. PMC: 4449161. DOI: 10.1371/journal.pone.0127738. View

Zhang H, Jia F, Li M, Yu F, Zhou B, Hao Q . Endophytic Bacillus strains isolated from alfalfa (Medicago sativa L.) seeds: enhancing the lifespan of Caenorhabditis elegans. Lett Appl Microbiol. 2018; 68(3):226-233. DOI: 10.1111/lam.13102. View

Dean R, van Kan J, Pretorius Z, Hammond-Kosack K, Di Pietro A, Spanu P . The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol. 2012; 13(4):414-30. PMC: 6638784. DOI: 10.1111/j.1364-3703.2011.00783.x. View

10.

PRESTON N, Morrell A . Reproducible results with the Gram stain. J Pathol Bacteriol. 1962; 84:241-3. DOI: 10.1002/path.1700840131. View

11.

Yamamoto S, Shiraishi S, Kawagoe Y, Mochizuki M, Suzuki S . Impact of Bacillus amyloliquefaciens S13-3 on control of bacterial wilt and powdery mildew in tomato. Pest Manag Sci. 2014; 71(5):722-7. DOI: 10.1002/ps.3837. View

12.

Radhakrishnan R, Hashem A, Abd Allah E . : A Biological Tool for Crop Improvement through Bio-Molecular Changes in Adverse Environments. Front Physiol. 2017; 8:667. PMC: 5592640. DOI: 10.3389/fphys.2017.00667. View

13.

Alvarez F, Castro M, Principe A, Borioli G, Fischer S, Mori G . The plant-associated Bacillus amyloliquefaciens strains MEP2 18 and ARP2 3 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease. J Appl Microbiol. 2011; 112(1):159-74. DOI: 10.1111/j.1365-2672.2011.05182.x. View

14.

Hajji-Hedfi L, Regaieg H, Larayedh A, Chihani N, Horrigue-Raouani N . Biological control of wilt disease complex on tomato crop caused by Meloidogyne javanica and Fusarium oxysporum f.sp. lycopersici by Verticillium leptobactrum. Environ Sci Pollut Res Int. 2017; 25(19):18297-18302. DOI: 10.1007/s11356-017-0233-6. View

15.

Raza W, Ling N, Zhang R, Huang Q, Xu Y, Shen Q . Success evaluation of the biological control of Fusarium wilts of cucumber, banana, and tomato since 2000 and future research strategies. Crit Rev Biotechnol. 2016; 37(2):202-212. DOI: 10.3109/07388551.2015.1130683. View

16.

Wu L, Wu H, Qiao J, Gao X, Borriss R . Novel Routes for Improving Biocontrol Activity of Bacillus Based Bioinoculants. Front Microbiol. 2015; 6:1395. PMC: 4674565. DOI: 10.3389/fmicb.2015.01395. View

17.

Pawlik M, Cania B, Thijs S, Vangronsveld J, Piotrowska-Seget Z . Hydrocarbon degradation potential and plant growth-promoting activity of culturable endophytic bacteria of Lotus corniculatus and Oenothera biennis from a long-term polluted site. Environ Sci Pollut Res Int. 2017; 24(24):19640-19652. PMC: 5570797. DOI: 10.1007/s11356-017-9496-1. View

18.

Fan B, Wang C, Song X, Ding X, Wu L, Wu H . FZB42 in 2018: The Gram-Positive Model Strain for Plant Growth Promotion and Biocontrol. Front Microbiol. 2018; 9:2491. PMC: 6198173. DOI: 10.3389/fmicb.2018.02491. View

19.

Nikolic I, Beric T, Dimkic I, Popovic T, Lozo J, Fira D . Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. J Appl Microbiol. 2018; 126(1):165-176. DOI: 10.1111/jam.14070. View

20.

Dunlap C, Kim S, Kwon S, Rooney A . is not a later heterotypic synonym of ; , subsp. and '' are later heterotypic synonyms of based on phylogenomics. Int J Syst Evol Microbiol. 2015; 66(3):1212-1217. DOI: 10.1099/ijsem.0.000858. View