Nephron Loss Detected by MRI Following Neonatal Acute Kidney Injury in Rabbits

Overview

Journal Pediatr Res

Specialties Biology
Pediatrics

Date 2019 Dec 6

PMID 31805577

Citations 22

Authors

Jennifer R Charlton

Edwin J Baldelomar

Kimberly A deRonde

Helen P Cathro

Nathan P Charlton

Stacey J Criswell

Dylan M Hyatt

Sejin Nam

Valeria Pearl

Kevin M Bennett

Affiliations

Soon will be listed here.

Abstract

Background: Acute kidney injury affects nearly 30% of preterm neonates in the intensive care unit. We aimed to determine whether nephrotoxin-induced AKI disrupted renal development assessed by imaging (CFE-MRI).

Methods: Neonatal New Zealand rabbits received indomethacin and gentamicin (AKI) or saline (control) for four days followed by cationic ferritin (CF) at six weeks. Ex vivo images were acquired using a gradient echo pulse sequence on 7 T MRI. Glomerular number (N) and apparent glomerular volume (aV) were determined. CF toxicity was assessed at two and 28 days in healthy rabbits.

Results: N was lower in the AKI group as compared to controls (74,034 vs 198,722, p < 0.01). aV was not different (AKI: 7.3 × 10 vs control: 6.2 × 10 mm, p = 0.69). AKI kidneys had a band of glomeruli distributed radially in the cortex that were undetectable by MRI. Following CF injection, there was no difference in body or organ weights except for the liver, and transient changes in serum iron, platelets and white blood cell count.

Conclusions: Brief nephrotoxin exposure during nephrogenesis results in fewer glomeruli and glomerular maldevelopment in a unique pattern detectable by MRI. Whole kidney evaluation by CFE-MRI may provide an important tool to understand the development of CKD following AKI.

Citing Articles

Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm Infants.

Carpenter J, Yarlagadda S, VandenHeuvel K, Ding L, Schuh M Kidney Int Rep. 2024; 9(2):436-450.

PMID: 38344733 PMC: 10851065. DOI: 10.1016/j.ekir.2023.10.032.

Assessment of Acute Kidney Injury using MRI.

Selby N, Francis S J Magn Reson Imaging. 2024; 61(1):25-41.

PMID: 38334370 PMC: 11645494. DOI: 10.1002/jmri.29281.

Characterizing post-branching nephrogenesis in the neonatal rabbit.

Schuh M, Yarlagadda S, Alkhudairy L, Preusse K, Kopan R Sci Rep. 2023; 13(1):19234.

PMID: 37932368 PMC: 10628296. DOI: 10.1038/s41598-023-46624-9.

Duration of Simultaneous Exposure to High-Risk and Lower-Risk Nephrotoxic Antimicrobials in the Neonatal Intensive Care Unit (NICU) and Future Adolescent Kidney Health.

Schiff A, Deines D, Jensen E, OConnell N, Perry C, Shaltout H J Pediatr. 2023; 264:113730.

PMID: 37722552 PMC: 10873056. DOI: 10.1016/j.jpeds.2023.113730.

Use of novel structural features to identify urinary biomarkers during acute kidney injury that predict progression to chronic kidney disease.

Charlton J, Li T, Wu T, deRonde K, Xu Y, Baldelomar E BMC Nephrol. 2023; 24(1):178.

PMID: 37331957 PMC: 10278294. DOI: 10.1186/s12882-023-03196-0.

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