Microbial Profile of Symptomatic Pericoronitis Lesions: a Cross-sectional Study

Overview

Journal J Appl Oral Sci

Specialty Dentistry

Date 2019 Dec 5

PMID 31800877

Citations 4

Authors

Marcus Heleno Borges Ribeiro

Paulo Cesar Ribeiro

Belen Retamal-Valdes

Magda Feres

Antonio Canabarro

Affiliations

Soon will be listed here.

Abstract

Objective: The microbial composition of pericoronitis (Pc) is still controversial; it is not yet clear if the microbial profile of these lesions is similar to the profile observed in periodontitis (Pd). Therefore, the aim of the present study was to describe the microbial profile of Pc lesions and compare it directly with that of subjects with Pd.

Methodology: Subjects with Pc and Pd were selected, and subgingival biofilm samples were collected from (i) third molars with symptomatic Pc (Pc-T), (ii) contralateral third molars without Pc (Pc-C) and (iii) teeth with a probing depth >3 mm from subjects with Pd. Counts and proportions of 40 bacterial species were evaluated using a checkerboard DNA-DNA hybridization technique.

Results: Twenty-six patients with Pc and 18 with Pd were included in the study. In general, higher levels of microorganisms were observed in Pd. Only Actinomyces oris and Eubacterium nodatum were present in higher mean counts in the Pc-T group in comparison with the Pc-C and Pd-C groups (p<0.05). The microbiota associated with Pc-T was similar to that found in Pc-C. Sites with Pc lesions had lower proportions of red complex in comparison with the Pd sites.

Conclusion: The microbiota of Pc is very diverse, but these lesions harbour lower levels of periodontal pathogens than Pd.

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References

Socransky S, Haffajee A, Smith C, Martin L, Haffajee J, Uzel N . Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol. 2004; 19(6):352-62. DOI: 10.1111/j.1399-302x.2004.00168.x. View

Zhu Y, Dashper S, Chen Y, Crawford S, Slakeski N, Reynolds E . Porphyromonas gingivalis and Treponema denticola synergistic polymicrobial biofilm development. PLoS One. 2013; 8(8):e71727. PMC: 3753311. DOI: 10.1371/journal.pone.0071727. View

Sencimen M, Saygun I, Gulses A, Bal V, Acikel C, Kubar A . Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR. Int Dent J. 2014; 64(4):200-5. PMC: 9376417. DOI: 10.1111/idj.12109. View

Singh P, Nath P, Bindra S, Rao S, Reddy K . The predictivity of mandibular third molar position as a risk indicator for pericoronitis: A prospective study. Natl J Maxillofac Surg. 2018; 9(2):215-221. PMC: 6251300. DOI: 10.4103/njms.NJMS_13_17. View

Socransky S, Haffajee A, Cugini M, Smith C, Kent Jr R . Microbial complexes in subgingival plaque. J Clin Periodontol. 1998; 25(2):134-44. DOI: 10.1111/j.1600-051x.1998.tb02419.x. View

Rajasuo A, Sihvonen O, Peltola M, Meurman J . Periodontal pathogens in erupting third molars of periodontally healthy subjects. Int J Oral Maxillofac Surg. 2007; 36(9):818-21. DOI: 10.1016/j.ijom.2007.05.001. View

Douglass A, Douglass J . Common dental emergencies. Am Fam Physician. 2003; 67(3):511-6. View

Teles R, Teles F, Frias-Lopez J, Paster B, Haffajee A . Lessons learned and unlearned in periodontal microbiology. Periodontol 2000. 2013; 62(1):95-162. PMC: 3912758. DOI: 10.1111/prd.12010. View

Ng H, Kin L, Dashper S, Slakeski N, Butler C, Reynolds E . Bacterial interactions in pathogenic subgingival plaque. Microb Pathog. 2015; 94:60-9. DOI: 10.1016/j.micpath.2015.10.022. View

10.

Papapanou P, Sanz M, Buduneli N, Dietrich T, Feres M, Fine D . Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018; 89 Suppl 1:S173-S182. DOI: 10.1002/JPER.17-0721. View

11.

Katsarou T, Kapsalas A, Souliou C, Stefaniotis T, Kalyvas D . Pericoronitis: A clinical and epidemiological study in greek military recruits. J Clin Exp Dent. 2019; 11(2):e133-e137. PMC: 6383902. DOI: 10.4317/jced.55383. View

12.

Jakovljevic A, Andric M, Knezevic A, Milicic B, Beljic-Ivanovic K, Perunovic N . Herpesviral-bacterial co-infection in mandibular third molar pericoronitis. Clin Oral Investig. 2016; 21(5):1639-1646. DOI: 10.1007/s00784-016-1955-4. View

13.

Socransky S, Smith C, Martin L, Paster B, Dewhirst F, Levin A . "Checkerboard" DNA-DNA hybridization. Biotechniques. 1994; 17(4):788-92. View

14.

Magraw C, Golden B, Phillips C, Tang D, Munson J, Nelson B . Pain with pericoronitis affects quality of life. J Oral Maxillofac Surg. 2014; 73(1):7-12. DOI: 10.1016/j.joms.2014.06.458. View

15.

Dashper S, Seers C, Tan K, Reynolds E . Virulence factors of the oral spirochete Treponema denticola. J Dent Res. 2010; 90(6):691-703. PMC: 3144123. DOI: 10.1177/0022034510385242. View

16.

Mansfield J, Campbell J, Bhandari A, Jesionowski A, Vickerman M . Molecular analysis of 16S rRNA genes identifies potentially periodontal pathogenic bacteria and archaea in the plaque of partially erupted third molars. J Oral Maxillofac Surg. 2012; 70(7):1507-14.e1-6. DOI: 10.1016/j.joms.2011.09.049. View

17.

Hallstrom H, Persson G, Stromberg U, Twetman S, Renvert S . Reproducibility of subgingival bacterial samples from patients with peri-implant mucositis. Clin Oral Investig. 2014; 19(5):1063-8. DOI: 10.1007/s00784-014-1324-0. View

18.

Labriola J, Mascaro J, Alpert B . The microbiologic flora of orofacial abscesses. J Oral Maxillofac Surg. 1983; 41(11):711-4. DOI: 10.1016/0278-2391(83)90186-6. View

19.

Perez-Chaparro P, Goncalves C, Figueiredo L, Faveri M, Lobao E, Tamashiro N . Newly identified pathogens associated with periodontitis: a systematic review. J Dent Res. 2014; 93(9):846-58. PMC: 4541103. DOI: 10.1177/0022034514542468. View

20.

Mineoka T, Awano S, Rikimaru T, Kurata H, Yoshida A, Ansai T . Site-specific development of periodontal disease is associated with increased levels of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque. J Periodontol. 2008; 79(4):670-6. DOI: 10.1902/jop.2008.070398. View