Alternative Pathway is Involved in Nitric Oxide-Enhanced Tolerance to Cadmium Stress in Barley Roots

Overview

Journal Plants (Basel)

Date 2019 Dec 5

PMID 31795459

Citations 8

Authors

Li He

Xiaomin Wang

Ruijun Feng

Qiang He

Shengwang Wang

Cuifang Liang

Lili Yan

Yurong Bi

Affiliations

Soon will be listed here.

Abstract

Alternative pathway (AP) has been widely accepted to be involved in enhancing tolerance to various environmental stresses. In this study, the role of AP in response to cadmium (Cd) stress in two barley varieties, highland barley (Kunlun14) and barley (Ganpi6), was investigated. Results showed that the malondialdehyde (MDA) content and electrolyte leakage (EL) level under Cd stress increased in two barley varieties. The expressions of alternative oxidase () genes (mainly ), AP capacity (V), and AOX protein amount were clearly induced more in Kunlun14 under Cd stress, and these parameters were further enhanced by applying sodium nitroprussid (SNP, a NO donor). Moreover, HO and O contents were raised in the Cd-treated roots of two barley varieties, but they were markedly relieved by exogenous SNP. However, this mitigating effect was aggravated by salicylhydroxamic acid (SHAM, an AOX inhibitor), suggesting that AP contributes to NO-enhanced Cd stress tolerance. Further study demonstrated that the effect of SHAM application on reactive oxygen species (ROS)-related scavenging enzymes and antioxidants was minimal. These observations showed that AP exerts an indispensable function in NO-enhanced Cd stress tolerance in two barley varieties. AP was mainly responsible for regulating the ROS accumulation to maintain the homeostasis of redox state.

Citing Articles

Nitric Oxide Mitigates the Deleterious Effects Caused by Infection of pv. and Modulates the Carbon Assimilation Process in Sweet Cherry under Water Stress.

Rubilar-Hernandez C, Alvarez-Maldini C, Pizarro L, Figueroa F, Villalobos-Gonzalez L, Pimentel P Plants (Basel). 2024; 13(10).

PMID: 38794433 PMC: 11125257. DOI: 10.3390/plants13101361.

Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes.

Deng X, Chen B, Luo Q, Zao X, Liu H, Li Y Front Nutr. 2022; 9:933068.

PMID: 35990339 PMC: 9389463. DOI: 10.3389/fnut.2022.933068.

UCP1 and AOX1a contribute to regulation of carbon and nitrogen metabolism and yield in Arabidopsis under low nitrogen stress.

Qiao X, Ruan M, Yu T, Cui C, Chen C, Zhu Y Cell Mol Life Sci. 2022; 79(1):69.

PMID: 34974624 PMC: 11072780. DOI: 10.1007/s00018-021-04036-w.

Trophic Transfer without Biomagnification of Cadmium in a Soybean-Dodder Parasitic System.

Chen B, Xu J, Wang X Plants (Basel). 2021; 10(12).

PMID: 34961161 PMC: 8703755. DOI: 10.3390/plants10122690.

Alternative Pathway Is Involved in Hydrogen Peroxide-Enhanced Cadmium Tolerance in Hulless Barley Roots.

He L, Wang X, Na X, Feng R, He Q, Wang S Plants (Basel). 2021; 10(11).

PMID: 34834692 PMC: 8622811. DOI: 10.3390/plants10112329.

References

Wanniarachchi V, Dametto L, Sweetman C, Shavrukov Y, Day D, Jenkins C . Alternative Respiratory Pathway Component Genes (AOX and ND) in Rice and Barley and Their Response to Stress. Int J Mol Sci. 2018; 19(3). PMC: 5877776. DOI: 10.3390/ijms19030915. View

Fan S, Fang X, Guan M, Ye Y, Lin X, Du S . Exogenous abscisic acid application decreases cadmium accumulation in Arabidopsis plants, which is associated with the inhibition of IRT1-mediated cadmium uptake. Front Plant Sci. 2015; 5:721. PMC: 4267193. DOI: 10.3389/fpls.2014.00721. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Amirsadeghi S, Robson C, McDonald A, Vanlerberghe G . Changes in plant mitochondrial electron transport alter cellular levels of reactive oxygen species and susceptibility to cell death signaling molecules. Plant Cell Physiol. 2006; 47(11):1509-19. DOI: 10.1093/pcp/pcl016. View

Zhang L, Chen Z, Zhu C . Endogenous nitric oxide mediates alleviation of cadmium toxicity induced by calcium in rice seedlings. J Environ Sci (China). 2012; 24(5):940-8. DOI: 10.1016/s1001-0742(11)60978-9. View

Domingos P, Prado A, Wong A, Gehring C, Feijo J . Nitric oxide: a multitasked signaling gas in plants. Mol Plant. 2015; 8(4):506-20. DOI: 10.1016/j.molp.2014.12.010. View

Jia H, Wang X, Dou Y, Liu D, Si W, Fang H . Hydrogen sulfide - cysteine cycle system enhances cadmium tolerance through alleviating cadmium-induced oxidative stress and ion toxicity in Arabidopsis roots. Sci Rep. 2016; 6:39702. PMC: 5177925. DOI: 10.1038/srep39702. View

Qian H, Chen W, Li J, Wang J, Zhou Z, Liu W . The effect of exogenous nitric oxide on alleviating herbicide damage in Chlorella vulgaris. Aquat Toxicol. 2009; 92(4):250-7. DOI: 10.1016/j.aquatox.2009.02.008. View

Asgher M, Per T, Masood A, Fatma M, Freschi L, Corpas F . Nitric oxide signaling and its crosstalk with other plant growth regulators in plant responses to abiotic stress. Environ Sci Pollut Res Int. 2016; 24(3):2273-2285. DOI: 10.1007/s11356-016-7947-8. View

10.

Ederli L, Morettini R, Borgogni A, Wasternack C, Miersch O, Reale L . Interaction between nitric oxide and ethylene in the induction of alternative oxidase in ozone-treated tobacco plants. Plant Physiol. 2006; 142(2):595-608. PMC: 1586042. DOI: 10.1104/pp.106.085472. View

11.

Qin W, Bazeille N, Henry E, Zhang B, Deprez E, Xi X . Mechanistic insight into cadmium-induced inactivation of the Bloom protein. Sci Rep. 2016; 6:26225. PMC: 4872126. DOI: 10.1038/srep26225. View

12.

Wang H, Huang J, Liang X, Bi Y . Involvement of hydrogen peroxide, calcium, and ethylene in the induction of the alternative pathway in chilling-stressed Arabidopsis callus. Planta. 2011; 235(1):53-67. DOI: 10.1007/s00425-011-1488-7. View

13.

Wang X, Ma R, Cui D, Cao Q, Shan Z, Jiao Z . Physio-biochemical and molecular mechanism underlying the enhanced heavy metal tolerance in highland barley seedlings pre-treated with low-dose gamma irradiation. Sci Rep. 2017; 7(1):14233. PMC: 5660250. DOI: 10.1038/s41598-017-14601-8. View

14.

Ribas-Carbo M, Berry J, Yakir D, Giles L, Robinson S, Lennon A . Electron Partitioning between the Cytochrome and Alternative Pathways in Plant Mitochondria. Plant Physiol. 1995; 109(3):829-837. PMC: 161383. DOI: 10.1104/pp.109.3.829. View

15.

Millenaar F, Gonzalez-Meler M, Fiorani F, Welschen R, Ribas-Carbo M, Siedow J . Regulation of alternative oxidase activity in six wild monocotyledonous species. An in vivo study at the whole root level. Plant Physiol. 2001; 126(1):376-87. PMC: 102311. DOI: 10.1104/pp.126.1.376. View

16.

Li S, Sun H, Li H, Luo J, Ma L . Assessment of cadmium bioaccessibility to predict its bioavailability in contaminated soils. Environ Int. 2016; 94:600-606. DOI: 10.1016/j.envint.2016.06.022. View

17.

Mur L, Mandon J, Persijn S, Cristescu S, Moshkov I, Novikova G . Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants. 2013; 5:pls052. PMC: 3560241. DOI: 10.1093/aobpla/pls052. View

18.

Lin A, Wang Y, Tang J, Xue P, Li C, Liu L . Nitric oxide and protein S-nitrosylation are integral to hydrogen peroxide-induced leaf cell death in rice. Plant Physiol. 2011; 158(1):451-64. PMC: 3252116. DOI: 10.1104/pp.111.184531. View

19.

Liu Y, Wu R, Wan Q, Xie G, Bi Y . Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean roots. Plant Cell Physiol. 2007; 48(3):511-22. DOI: 10.1093/pcp/pcm020. View

20.

Planchet E, Gupta K, Sonoda M, Kaiser W . Nitric oxide emission from tobacco leaves and cell suspensions: rate limiting factors and evidence for the involvement of mitochondrial electron transport. Plant J. 2005; 41(5):732-43. DOI: 10.1111/j.1365-313X.2005.02335.x. View