Inhibition of Porcine Aminopeptidase M (pAMP) by the Pentapeptide Microginins

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Dec 5

PMID 31795383

Citations 4

Authors

Glaucio Monteiro Ferreira

Thales Kronenberger

Eryka Costa de Almeida

Joseane Sampaio

Clelia Ferreira Terra

Ernani Pinto

Gustavo Henrique Goulart Trossini

Affiliations

Soon will be listed here.

Abstract

Aminopeptidase M (AMP) inhibition is of interest for several diseases, such as highly vascularized cancer types. AMP can be inhibited by linear pentapeptides isolated from LTPNA08 (MG7XX). Porcine AMP inhibition-a model for human AMP-activity was spectrophotometrically measured by the formation of p-nitroanilide from L-leucine-p-nitroanilide substrate by AMP. AMP inhibition by MG770 exhibited comparable inhibition levels to amastatin (IC values: 1.20 ± 0.1 μM and 0.98 ± 0.1 μM, respectively), while MG756 was slightly less potent (with IC values of 3.26 ± 0.5 μM). Molecular modelling suggests a potential binding mode, based on the interaction with the Zn cofactor, where MG770's extra methyl group contributes to the disturbance of the Zn cofactor complex and highlights the importance of hydrophobicity for the site.

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