Biodegradable Drug-Delivery Peptide Nanocapsules

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2019 Dec 3

PMID 31788640

Citations 6

Authors

Emily M Wessel

John M Tomich

Richard B Todd

Affiliations

Soon will be listed here.

Abstract

Branched amphiphilic peptide capsules (BAPCs) are an efficient transport system that can deliver nucleic acids, small proteins, and solutes. The ability of BAPCs to break down is essential to their adoption as a delivery vehicle for human and agricultural applications. Until now, however, BAPCs were shown to be inert to mammalian degradation systems. Here, we demonstrate, using BAPCs encapsulating the toxic urea analogue thiourea, that the common soil fungus can degrade BAPCs. We provide evidence that this degradation is extracellular through the action of secreted proteases. Our data indicate that BAPCs are likely biodegradable in the environment.

Citing Articles

The potential of HBV cure: an overview of CRISPR-mediated HBV gene disruption.

Yao Z, Schank M, Zhao J, El Gazzar M, Wang L, Zhang Y Front Genome Ed. 2024; 6:1467449.

PMID: 39444780 PMC: 11496132. DOI: 10.3389/fgeed.2024.1467449.

Biodistribution Analysis of Peptide-Coated Magnetic Iron Nanoparticles: A Simple and Quantitative Method.

Natarajan P, Horak K, Rowe J, Yoon S, Lingo J, Tomich J Mol Pharm. 2024; 21(2):970-981.

PMID: 38206824 PMC: 10918533. DOI: 10.1021/acs.molpharmaceut.3c01080.

Synthetic gRNA/Cas9 ribonucleoprotein targeting HBV DNA inhibits viral replication.

Zhang J, Zhang Y, Khanal S, Cao D, Zhao J, Dang X J Med Virol. 2023; 95(7):e28952.

PMID: 37455550 PMC: 10977344. DOI: 10.1002/jmv.28952.

Understanding the influence of experimental factors on bio-interactions of nanoparticles: Towards improving correlation between in vitro and in vivo studies.

Natarajan P, Tomich J Arch Biochem Biophys. 2020; 694:108592.

PMID: 32971033 PMC: 7503072. DOI: 10.1016/j.abb.2020.108592.

Synergies with and Resistance to Membrane-Active Peptides.

Kmeck A, Tancer R, Ventura C, Wiedman G Antibiotics (Basel). 2020; 9(9).

PMID: 32961656 PMC: 7559582. DOI: 10.3390/antibiotics9090620.

References

Barros S, Avila L, Whitaker S, Wilkinson K, Sukthankar P, Beltrao E . Branched Amphipathic Peptide Capsules: Different Ratios of the Two Constituent Peptides Direct Distinct Bilayer Structures, Sizes, and DNA Transfection Efficiency. Langmuir. 2017; 33(28):7096-7104. DOI: 10.1021/acs.langmuir.7b00912. View

Cohen B . Regulation of intracellular and extracellular neutral and alkaline proteases in Aspergillus nidulans. J Gen Microbiol. 1973; 79(2):311-20. DOI: 10.1099/00221287-79-2-311. View

Katz M, Flynn P, vanKuyk P, Cheetham B . Mutations affecting extracellular protease production in the filamentous fungus Aspergillus nidulans. Mol Gen Genet. 1996; 250(6):715-24. DOI: 10.1007/BF02172983. View

Barros S, Whitaker S, Sukthankar P, Avila L, Gudlur S, Warner M . A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules. Arch Biochem Biophys. 2016; 596:22-42. PMC: 4841695. DOI: 10.1016/j.abb.2016.02.027. View

Hynes M, Pateman J . The use of amides as nitrogen sources by Aspergillus nidulans. J Gen Microbiol. 1970; 63(3):317-24. DOI: 10.1099/00221287-63-3-317. View

Sukthankar P, Whitaker S, Garcia M, Herrera A, Boatwright M, Prakash O . Thermally induced conformational transitions in nascent branched amphiphilic peptide capsules. Langmuir. 2015; 31(10):2946-55. DOI: 10.1021/la504381y. View

Avila L, Aps L, Sukthankar P, Ploscariu N, Gudlur S, Simo L . Branched amphiphilic cationic oligopeptides form peptiplexes with DNA: a study of their biophysical properties and transfection efficiency. Mol Pharm. 2015; 12(3):706-15. DOI: 10.1021/mp500524s. View

Grunbacher A, Throm T, Seidel C, Gutt B, Rohrig J, Strunk T . Six hydrophobins are involved in hydrophobin rodlet formation in Aspergillus nidulans and contribute to hydrophobicity of the spore surface. PLoS One. 2014; 9(4):e94546. PMC: 3983194. DOI: 10.1371/journal.pone.0094546. View

Katz M, Gray K, Cheetham B . The Aspergillus nidulans xprG (phoG) gene encodes a putative transcriptional activator involved in the response to nutrient limitation. Fungal Genet Biol. 2006; 43(3):190-9. DOI: 10.1016/j.fgb.2005.12.001. View

10.

Pateman J, Kinghorn J, Dunn E, Forbes E . Ammonium regulation in Aspergillus nidulans. J Bacteriol. 1973; 114(3):943-50. PMC: 285348. DOI: 10.1128/jb.114.3.943-950.1973. View

11.

Zablotowicz R, Abbas H, Locke M . Population ecology of Aspergillus flavus associated with Mississippi Delta soils. Food Addit Contam. 2007; 24(10):1102-8. DOI: 10.1080/02652030701546198. View

12.

Todd R, Davis M, Hynes M . Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction. Nat Protoc. 2007; 2(4):811-21. DOI: 10.1038/nprot.2007.112. View

13.

Grishkan I . Thermotolerant mycobiota of Israeli soils. J Basic Microbiol. 2017; 58(1):30-40. DOI: 10.1002/jobm.201700517. View

14.

Klich M . Biogeography of Aspergillus species in soil and litter. Mycologia. 2010; 94(1):21-7. View

15.

Kito M, Takimoto R, Yoshida T, Nagasawa T . Purification and characterization of an epsilon-poly-L-lysine-degrading enzyme from an epsilon-poly-L-lysine-producing strain of Streptomyces albulus. Arch Microbiol. 2002; 178(5):325-30. DOI: 10.1007/s00203-002-0459-6. View

16.

Gudlur S, Sukthankar P, Gao J, Avila L, Hiromasa Y, Chen J . Peptide nanovesicles formed by the self-assembly of branched amphiphilic peptides. PLoS One. 2012; 7(9):e45374. PMC: 3445502. DOI: 10.1371/journal.pone.0045374. View

17.

Avila L, Chandrasekar R, Wilkinson K, Balthazor J, Heerman M, Bechard J . Delivery of lethal dsRNAs in insect diets by branched amphiphilic peptide capsules. J Control Release. 2018; 273:139-146. PMC: 6984438. DOI: 10.1016/j.jconrel.2018.01.010. View

18.

Sukthankar P, Avila L, Whitaker S, Iwamoto T, Morgenstern A, Apostolidis C . Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes. Biochim Biophys Acta. 2014; 1838(9):2296-305. PMC: 4082727. DOI: 10.1016/j.bbamem.2014.02.005. View

19.

ElHady A, Gine A, Topalovic O, Jacquiod S, Sorensen S, Sorribas F . Microbiomes associated with infective stages of root-knot and lesion nematodes in soil. PLoS One. 2017; 12(5):e0177145. PMC: 5417685. DOI: 10.1371/journal.pone.0177145. View

20.

Horn B . Relationship between soil densities of Aspergillus species and colonization of wounded peanut seeds. Can J Microbiol. 2006; 52(10):951-60. DOI: 10.1139/w06-050. View