Definition of the Anti-inflammatory Oligosaccharides Derived From the Galactosaminogalactan (GAG) From

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2019 Nov 30

PMID 31781511

Citations 13

Authors

Markus Gressler

Christoph Heddergott

Ines C NGo

Giorgia Renga

Vasilis Oikonomou

Silvia Moretti

Bernadette Coddeville

Joana Gaifem

Ricardo Silvestre

Luigina Romani

Jean-Paul Latge

Thierry Fontaine

Affiliations

Soon will be listed here.

Abstract

Galactosaminogalactan (GAG) is an insoluble aminosugar polymer produced by and has anti-inflammatory properties. Here, the minimum glycosidic sequences required for the induction of IL-1Ra by peripheral blood mononuclear cells (PBMCs) was investigated. Using chemical degradation of native GAG to isolate soluble oligomers, we have found that the de--acetylation of galactosamine residues and the size of oligomer are critical for the immune response. A minimal oligomer size of 20 galactosamine residues is required for the anti-inflammatory response but the presence of galactose residues is not necessary. In a Dextran sulfate induced colitis mouse model, a fraction of de--acetylated oligomers of 13 < dp < 20 rescue inflammatory damage like the native GAG polymer in an IL-1Ra dependent pathway. Our results demonstrate the therapeutic suitability of water-soluble GAG oligosaccharides in IL-1 mediated hyper-inflammatory diseases and suggest that α-1,4-galactosamine oligomers chemically synthesized could represent new anti-inflammatory glycodrugs.

Citing Articles

Interplay between host humoral pattern recognition molecules controls undue immune responses against Aspergillus fumigatus.

Delliere S, Chauvin C, Wong S, Gressler M, Possetti V, Parente R Nat Commun. 2024; 15(1):6966.

PMID: 39138196 PMC: 11322389. DOI: 10.1038/s41467-024-51047-9.

CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan.

Heilig L, Natasha F, Trinks N, Aimanianda V, Wong S, Fontaine T PLoS Pathog. 2024; 20(6):e1012315.

PMID: 38889192 PMC: 11216564. DOI: 10.1371/journal.ppat.1012315.

Can Display Persistence to the Fungicidal Drug Voriconazole.

Scott J, Valero C, Mato-Lopez A, Donaldson I, Roldan A, Chown H Microbiol Spectr. 2023; :e0477022.

PMID: 36912663 PMC: 10100717. DOI: 10.1128/spectrum.04770-22.

Tackling Inflammatory Bowel Diseases: Targeting Proinflammatory Cytokines and Lymphocyte Homing.

Song Y, Yuan M, Xu Y, Xu H Pharmaceuticals (Basel). 2022; 15(9).

PMID: 36145301 PMC: 9502105. DOI: 10.3390/ph15091080.

An Alanine Aminotransferase Is Required for Biofilm-Specific Resistance of Aspergillus fumigatus to Echinocandin Treatment.

Kerkaert J, Le Mauff F, Wucher B, Beattie S, Vesely E, Sheppard D mBio. 2022; 13(2):e0293321.

PMID: 35254131 PMC: 9040767. DOI: 10.1128/mbio.02933-21.

References

Salman H, Bergman M, Djaldetti M, Orlin J, Bessler H . Citrus pectin affects cytokine production by human peripheral blood mononuclear cells. Biomed Pharmacother. 2008; 62(9):579-82. DOI: 10.1016/j.biopha.2008.07.058. View

Marx A, Backes C, Meese E, Lenhof H, Keller A . EDISON-WMW: Exact Dynamic Programing Solution of the Wilcoxon-Mann-Whitney Test. Genomics Proteomics Bioinformatics. 2016; 14(1):55-61. PMC: 4792850. DOI: 10.1016/j.gpb.2015.11.004. View

Smeekens S, Gresnigt M, Becker K, Cheng S, Netea S, Jacobs L . An anti-inflammatory property of Candida albicans β-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism. Cytokine. 2014; 71(2):215-22. PMC: 4437193. DOI: 10.1016/j.cyto.2014.10.013. View

Gresnigt M, Bozza S, Becker K, Joosten L, Abdollahi-Roodsaz S, van der Berg W . A polysaccharide virulence factor from Aspergillus fumigatus elicits anti-inflammatory effects through induction of Interleukin-1 receptor antagonist. PLoS Pathog. 2014; 10(3):e1003936. PMC: 3946377. DOI: 10.1371/journal.ppat.1003936. View

Wang H, Dong J, Shi P, Liu J, Zuo L, Li Y . Anti-mouse CD52 monoclonal antibody ameliorates intestinal epithelial barrier function in interleukin-10 knockout mice with spontaneous chronic colitis. Immunology. 2014; 144(2):254-62. PMC: 4298419. DOI: 10.1111/imm.12366. View

Poutsiaka D, Mengozzi M, Vannier E, Sinha B, Dinarello C . Cross-linking of the beta-glucan receptor on human monocytes results in interleukin-1 receptor antagonist but not interleukin-1 production. Blood. 1993; 82(12):3695-700. View

Lee M, Liu H, Barker B, Snarr B, Gravelat F, Al Abdallah Q . The Fungal Exopolysaccharide Galactosaminogalactan Mediates Virulence by Enhancing Resistance to Neutrophil Extracellular Traps. PLoS Pathog. 2015; 11(10):e1005187. PMC: 4619649. DOI: 10.1371/journal.ppat.1005187. View

Tamura J, Abe T, Hasegawa K, Kadowaki K . The Mode of Action of Endo α-1,4 Polygalactosaminidase from Pseudomonas sp. 881 on Galactosaminooligosaccharides. Biosci Biotechnol Biochem. 2016; 56(3):380-3. DOI: 10.1271/bbb.56.380. View

Popov S, Ovodov Y . Polypotency of the immunomodulatory effect of pectins. Biochemistry (Mosc). 2013; 78(7):823-35. DOI: 10.1134/S0006297913070134. View

10.

Fontaine T, Delangle A, Simenel C, Coddeville B, van Vliet S, van Kooyk Y . Galactosaminogalactan, a new immunosuppressive polysaccharide of Aspergillus fumigatus. PLoS Pathog. 2011; 7(11):e1002372. PMC: 3213105. DOI: 10.1371/journal.ppat.1002372. View

11.

Gravelat F, Beauvais A, Liu H, Lee M, Snarr B, Chen D . Aspergillus galactosaminogalactan mediates adherence to host constituents and conceals hyphal β-glucan from the immune system. PLoS Pathog. 2013; 9(8):e1003575. PMC: 3749958. DOI: 10.1371/journal.ppat.1003575. View

12.

Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V . A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol. 2014; 192(11):5332-42. DOI: 10.4049/jimmunol.1303180. View

13.

Beaussart A, El-Kirat-Chatel S, Fontaine T, Latge J, Dufrene Y . Nanoscale biophysical properties of the cell surface galactosaminogalactan from the fungal pathogen Aspergillus fumigatus. Nanoscale. 2015; 7(36):14996-5004. DOI: 10.1039/c5nr04399a. View

14.

McNamee E, Masterson J, Jedlicka P, McManus M, Grenz A, Collins C . Interleukin 37 expression protects mice from colitis. Proc Natl Acad Sci U S A. 2011; 108(40):16711-6. PMC: 3189085. DOI: 10.1073/pnas.1111982108. View

15.

Alvarez F . The effect of chitin size, shape, source and purification method on immune recognition. Molecules. 2014; 19(4):4433-51. PMC: 6271096. DOI: 10.3390/molecules19044433. View

16.

Vo T, Kim S . Marine-derived polysaccharides for regulation of allergic responses. Adv Food Nutr Res. 2014; 73:1-13. DOI: 10.1016/B978-0-12-800268-1.00001-9. View

17.

Hamer S, Cord-Landwehr S, Biarnes X, Planas A, Waegeman H, Moerschbacher B . Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases. Sci Rep. 2015; 5:8716. PMC: 4346795. DOI: 10.1038/srep08716. View

18.

Lever M . A new reaction for colorimetric determination of carbohydrates. Anal Biochem. 1972; 47(1):273-9. DOI: 10.1016/0003-2697(72)90301-6. View

19.

Lee M, Geller A, Bamford N, Liu H, Gravelat F, Snarr B . Deacetylation of Fungal Exopolysaccharide Mediates Adhesion and Biofilm Formation. mBio. 2016; 7(2):e00252-16. PMC: 4817252. DOI: 10.1128/mBio.00252-16. View

20.

Bueter C, Lee C, Rathinam V, Healy G, Taron C, Specht C . Chitosan but not chitin activates the inflammasome by a mechanism dependent upon phagocytosis. J Biol Chem. 2011; 286(41):35447-35455. PMC: 3195641. DOI: 10.1074/jbc.M111.274936. View