MiR29a and MiR378b Influence CpG-Stimulated Dendritic Cells and Regulate CGAS/STING Pathway

Overview

Journal Vaccines (Basel)

Date 2019 Nov 30

PMID 31779082

Citations 10

Authors

Abid Ullah Shah

Yanan Cao

Naila Siddique

Jian Lin

Qian Yang

Affiliations

Soon will be listed here.

Abstract

The Cytosine-phosphate-guanosine (CpG) motif, which is specifically recognized intracellularly by dendritic cells (DCs), plays a crucial role in regulating the innate immune response. MicroRNAs (miRNAs) can strongly influence the antigen-presenting ability of DCs. In this study, we examine the action of miRNAs on CpG-stimulated and control DCs, as well as their effect on cyclic guanosine monophosphate-adenosine monophosphate (GMP-AMP) synthase () and the stimulator of interferon genes () signal pathway. Firstly, we selected miRNAs (miR-29a and miR-378b) based on expression in CpG-stimulated mouse bone marrow-derived dendritic cells (BMDCs). Secondly, we investigated the functions of miR-29a and miR-378b on CpG-stimulated and unstimulated BMDCs. The results showed that miR-29a and miR-378b increased expression of both the immunoregulatory DC surface markers (CD86 and CD40) and the immunosuppressive molecule CD273 by DCs. Thirdly, cytokine detection revealed that both miR-29a and miR-378b enhanced interferon-β (IFN-β) expression while suppressing tumor necrosis factor-α (TNF-α) production. Finally, our results suggest that miR-378b can bind TANK-binding kinase binding protein 1 () to activate the cGAS/STING signaling pathway. By contrast, miR-29a targeted interferon regulatory factor 7 () and promoted the expression of STING. Together, our results provide insight into the molecular mechanism of miRNA induction by CpG to regulate DC function.

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