Identification of Mycobacterium Abscessus Species and Subspecies Using the Cas12a/sgRNA-based Nucleic Acid Detection Platform

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2019 Nov 29

PMID 31776874

Citations 8

Authors

Guohui Xiao

Su Zhang

Zhihang Liang

Guanqiang Li

Mutong Fang

Yaya Liu

Juanjuan Zhang

Min Ou

Xing He

Tianyu Zhang

Changchun Zeng

Lei Liu

Guoliang Zhang

Affiliations

Soon will be listed here.

Abstract

The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify M. abscessus species and subspecies. By designing specific sgRNA probes targeting rpoB and erm(41), we demonstrated that M. abscessus could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical M. abscessus isolates were identified, 18 as M. abscessus subsp. abscessus and 20 as M. abscessus subsp. massiliense. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of M. abscessus species and subspecies.

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