The Mechanism Research of Non-Smad Dependent TAK1 Signaling Pathway in the Treatment of Bone Defects by Recombination BMP-2-loaded Hollow Hydroxyapatite Microspheres/chitosan Composite

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2019 Nov 29

PMID 31776786

Citations 2

Authors

Jingtang Li

Shilang Xiong

Linghua Ding

Jianhua Zeng

Peng Qiu

Jianguo Zhou

Xingen Liao

Long Xiong

Affiliations

Soon will be listed here.

Abstract

Aims: The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.

Methods: Firstly, the biological activity of rhBMP-2 released from the complex was investigated. Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed. Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).

Results: RhBMP-2 could be loaded and released from the complex in bioactive form. Additionally, the complex provided a prolonged period of time compared with HA/CS scaffolds. Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group. In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group. In the three-point bending test, load values in p38 inhibitor group decreased. In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group. In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.

Conclusion: The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.

Citing Articles

Bioactive Porous Composite Implant Guides Mesenchymal Stem Cell Differentiation and Migration to Accelerate Bone Reconstruction.

Wang S, Xia D, Dou W, Chen A, Xu S Int J Nanomedicine. 2024; 19:12111-12127.

PMID: 39583325 PMC: 11586122. DOI: 10.2147/IJN.S479893.

Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects.

Zeng J, Xiong S, Zhou J, Wei P, Guo K, Wang F Int J Nanomedicine. 2023; 18:3509-3534.

PMID: 37404852 PMC: 10317543. DOI: 10.2147/IJN.S408905.

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