Silencing LncRNA FOXD2-AS1 Inhibits Proliferation, Migration, Invasion and Drug Resistance of Drug-resistant Glioma Cells and Promotes Their Apoptosis Via MicroRNA-98-5p/CPEB4 Axis

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2019 Nov 27

PMID 31770107

Citations 30

Authors

Naibing Gu

Xinlai Wang

Zhengli Di

Jing Xiong

Yue Ma

Yue Yan

Yihua Qian

Quanzeng Zhang

Jia Yu

Affiliations

Soon will be listed here.

Abstract

Objective: This study was conducted to elucidate the long non-coding RNA FOXD2-AS1 (lncRNA FOXD2-AS1) expression in glioma and its mechanism on the biological features of glioma cells and the drug resistance of temozolomide (TMZ).

Results: Highly expressed FOXD2-AS1 was found in glioma. There was more powerful chemotherapeutic resistance of TMZ resistant cell lines than that of the parent cell lines. Silence of FOXD2-AS1 suppressed proliferation and drug resistance and promoted apoptosis of drug-resistant glioma cells. Overexpressed FOXD2-AS1 presented an opposite trend. FOXD2-AS1 could be used as a competing endogenous RNA to adsorb miR-98-5p, thereby up-regulating CPEB4.

Conclusion: Our study suggests that down-regulated FOXD2-AS1 repressed invasion, proliferation, migration and drug resistance of drug-resistant glioma cells while stimulating their apoptosis via increasing miR-98-5p and inhibiting CPEB4 expression.

Methods: FOXD2-AS1, microRNA-98-5p (miR-98-5p) and cytoplasmic polyadenylation element binding (CPEB4) expression in glioma tissues were tested. Expression of E-cadherin, N-cadherin and Vimentin in glioma cells were explored. A series of assays were conducted to detect the function of FOXD2-AS1 in migration, proliferation, apoptosis, and invasion of glioma cells. Changes in drug-resistance of cells under TMZ treatment were examined, and tumor formation in nude mice was performed to test the changes of drug resistance .

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References

Fan Y, Ye M, Wu L, Lv S, Wu M, Xiao B . Overexpression of miR-98 inhibits cell invasion in glioma cell lines via downregulation of IKKε. Eur Rev Med Pharmacol Sci. 2015; 19(19):3593-604. View

An Q, Zhou L, Xu N . Long noncoding RNA FOXD2-AS1 accelerates the gemcitabine-resistance of bladder cancer by sponging miR-143. Biomed Pharmacother. 2018; 103:415-420. DOI: 10.1016/j.biopha.2018.03.138. View

Villa C, Miquel C, Mosses D, Bernier M, Di Stefano A . The 2016 World Health Organization classification of tumours of the central nervous system. Presse Med. 2018; 47(11-12 Pt 2):e187-e200. DOI: 10.1016/j.lpm.2018.04.015. View

Ni W, Xia Y, Bi Y, Wen F, Hu D, Luo L . FoxD2-AS1 promotes glioma progression by regulating miR-185-5P/HMGA2 axis and PI3K/AKT signaling pathway. Aging (Albany NY). 2019; 11(5):1427-1439. PMC: 6428107. DOI: 10.18632/aging.101843. View

Wu Q, Shi M, Meng W, Wang Y, Hui P, Ma J . Long noncoding RNA FOXD3-AS1 promotes colon adenocarcinoma progression and functions as a competing endogenous RNA to regulate SIRT1 by sponging miR-135a-5p. J Cell Physiol. 2019; 234(12):21889-21902. DOI: 10.1002/jcp.28752. View

Shen F, Chang H, Gao G, Zhang B, Li X, Jin B . Long noncoding RNA FOXD2-AS1 promotes glioma malignancy and tumorigenesis via targeting miR-185-5p/CCND2 axis. J Cell Biochem. 2018; 120(6):9324-9336. DOI: 10.1002/jcb.28208. View

Ostermann S, Csajka C, Buclin T, Leyvraz S, Lejeune F, Decosterd L . Plasma and cerebrospinal fluid population pharmacokinetics of temozolomide in malignant glioma patients. Clin Cancer Res. 2004; 10(11):3728-36. DOI: 10.1158/1078-0432.CCR-03-0807. View

Liao Y, Shen L, Zhao H, Liu Q, Fu J, Guo Y . LncRNA CASC2 Interacts With miR-181a to Modulate Glioma Growth and Resistance to TMZ Through PTEN Pathway. J Cell Biochem. 2017; 118(7):1889-1899. DOI: 10.1002/jcb.25910. View

Liu X, Fu Q, Li S, Liang N, Li F, Li C . LncRNA FOXD2-AS1 Functions as a Competing Endogenous RNA to Regulate TERT Expression by Sponging miR-7-5p in Thyroid Cancer. Front Endocrinol (Lausanne). 2019; 10:207. PMC: 6463795. DOI: 10.3389/fendo.2019.00207. View

10.

Yang B, Fu X, Hao J, Sun J, Li Z, Li H . PAXX Participates in Base Excision Repair via Interacting with Pol β and Contributes to TMZ Resistance in Glioma Cells. J Mol Neurosci. 2018; 66(2):214-221. PMC: 6182633. DOI: 10.1007/s12031-018-1157-4. View

11.

Ochiai Y, Sano E, Okamoto Y, Yoshimura S, Makita K, Yamamuro S . Efficacy of ribavirin against malignant glioma cell lines: Follow-up study. Oncol Rep. 2017; 39(2):537-544. PMC: 5783620. DOI: 10.3892/or.2017.6149. View

12.

Yang X, Duan B, Zhou X . Long non-coding RNA FOXD2-AS1 functions as a tumor promoter in colorectal cancer by regulating EMT and Notch signaling pathway. Eur Rev Med Pharmacol Sci. 2017; 21(16):3586-3591. View

13.

Yang Y, Zhang Y, Chen B, Ding L, Mu Z, Li Y . Elevation of circular RNA circ-POSTN facilitates cell growth and invasion by sponging miR-1205 in glioma. J Cell Biochem. 2019; 120(10):16567-16574. DOI: 10.1002/jcb.28916. View

14.

Chen Z, Cheng Q, Ma Z, Xi H, Peng R, Jiang B . Overexpression of RKIP inhibits cell invasion in glioma cell lines through upregulation of miR-98. Biomed Res Int. 2014; 2013:695179. PMC: 3874320. DOI: 10.1155/2013/695179. View

15.

Wang L, Guo S, Zhang H . MiR-98 Promotes Apoptosis of Glioma Cells via Suppressing IKBKE/NF-κB Pathway. Technol Cancer Res Treat. 2018; 16(6):1226-1234. PMC: 5762096. DOI: 10.1177/1533034617745761. View

16.

Su H, Zou D, Sun Y, Dai Y . Hypoxia-associated circDENND2A promotes glioma aggressiveness by sponging miR-625-5p. Cell Mol Biol Lett. 2019; 24:24. PMC: 6446274. DOI: 10.1186/s11658-019-0149-x. View

17.

Dong H, Cao W, Xue J . Long noncoding FOXD2-AS1 is activated by CREB1 and promotes cell proliferation and metastasis in glioma by sponging miR-185 through targeting AKT1. Biochem Biophys Res Commun. 2018; 508(4):1074-1081. DOI: 10.1016/j.bbrc.2018.12.050. View

18.

Ostrom Q, Bauchet L, Davis F, Deltour I, Fisher J, Langer C . The epidemiology of glioma in adults: a "state of the science" review. Neuro Oncol. 2014; 16(7):896-913. PMC: 4057143. DOI: 10.1093/neuonc/nou087. View

19.

Hu W, Yang Y, Xi S, Sai K, Su D, Zhang X . Expression of CPEB4 in Human Glioma and Its Correlations With Prognosis. Medicine (Baltimore). 2015; 94(27):e979. PMC: 4504610. DOI: 10.1097/MD.0000000000000979. View

20.

Xu X, Bao Z, Liu Y, Ji J, Liu N . MicroRNA-98 Attenuates Cell Migration and Invasion in Glioma by Directly Targeting Pre-B Cell Leukemia Homeobox 3. Cell Mol Neurobiol. 2017; 37(8):1359-1371. PMC: 11482209. DOI: 10.1007/s10571-017-0466-4. View