A Luminescent MicroRNA Nanoprobe Based on the Target-triggered Release of an Iridium(III)-solvent Complex from Mesoporous Silica Nanoparticles

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2019 Nov 27

PMID 31768640

Authors

Xiaomin Tang

Kaimei Fan

Baohan Qu

Lihua Lu

Affiliations

Soon will be listed here.

Abstract

A luminescent microRNA nanoprobe based on the target-triggered Ir(III)-solvent complex release has been fabricated. The complex is initially embedded into mesoporous silica nanoparticles (MSNs), and then is capped by single-stranded (ss) DNA. In the presence of the target microRNA, the ssDNA hybridize with the microRNA forming a rigid DNA/RNA heteroduplexes and leaving the surface of MSN. Thus, the capped Ir(III) solvent complex is released and re-coordinated with histidine (His) to form a new luminescent complex. The luminescence intensity of the nascent complex (with excitation/emission maxima at 340/570 nm) is positively correlated with the concentrations of the target microRNA in the range from 0.05 to 2 nM, and the detection limit of microRNA is estimated as 0.2 pM (S/N = 3). The ability of this nanoprobe to detect microRNA in cell extract further demonstrates its potential in practical application. Graphical abstractSchematic of a luminescent microRNA nanoprobe based on the target-triggered release of an Ir(III)-solvent complex from mesoporous silica nanoparticles.

References

Chen Y, Zhang W, Huang K, Zheng M, Mao Y . An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA-RNA hybridization. Analyst. 2017; 142(24):4843-4851. DOI: 10.1039/c7an01244f. View

Li C, Lu W, Zhu M, Tang B . Development of Visible-Light Induced Photoelectrochemical Platform Based on Cyclometalated Iridium(III) Complex for Bioanalysis. Anal Chem. 2017; 89(20):11098-11106. DOI: 10.1021/acs.analchem.7b03229. View

Xie Y, Lin X, Huang Y, Pan R, Zhu Z, Zhou L . Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres. Chem Commun (Camb). 2015; 51(11):2156-8. DOI: 10.1039/c4cc08912j. View

Vivero-Escoto J, Slowing I, Trewyn B, Lin V . Mesoporous silica nanoparticles for intracellular controlled drug delivery. Small. 2010; 6(18):1952-67. DOI: 10.1002/smll.200901789. View

Liao R, He K, Chen C, Chen X, Cai C . Double-Strand Displacement Biosensor and Quencher-Free Fluorescence Strategy for Rapid Detection of MicroRNA. Anal Chem. 2016; 88(8):4254-8. DOI: 10.1021/acs.analchem.5b04154. View

Tu W, Cao H, Zhang L, Bao J, Liu X, Dai Z . Dual Signal Amplification Using Gold Nanoparticles-Enhanced Zinc Selenide Nanoflakes and P19 Protein for Ultrasensitive Photoelectrochemical Biosensing of MicroRNA in Cell. Anal Chem. 2016; 88(21):10459-10465. DOI: 10.1021/acs.analchem.6b02381. View

Dong H, Lei J, Ding L, Wen Y, Ju H, Zhang X . MicroRNA: function, detection, and bioanalysis. Chem Rev. 2013; 113(8):6207-33. DOI: 10.1021/cr300362f. View

Yuan Z, Zhou Y, Gao S, Cheng Y, Li Z . Homogeneous and sensitive detection of microRNA with ligase chain reaction and lambda exonuclease-assisted cationic conjugated polymer biosensing. ACS Appl Mater Interfaces. 2014; 6(9):6181-5. DOI: 10.1021/am500883q. View

Zhang P, Wu X, Yuan R, Chai Y . An "off-on" electrochemiluminescent biosensor based on DNAzyme-assisted target recycling and rolling circle amplifications for ultrasensitive detection of microRNA. Anal Chem. 2015; 87(6):3202-7. DOI: 10.1021/ac504455z. View

10.

Cha W, Fan R, Miao Y, Zhou Y, Qin C, Shan X . MicroRNAs as novel endogenous targets for regulation and therapeutic treatments. Medchemcomm. 2018; 9(3):396-408. PMC: 6072415. DOI: 10.1039/c7md00285h. View

11.

Climent E, Martinez-Manez R, Sancenon F, Marcos M, Soto J, Maquieira A . Controlled delivery using oligonucleotide-capped mesoporous silica nanoparticles. Angew Chem Int Ed Engl. 2010; 49(40):7281-3. DOI: 10.1002/anie.201001847. View

12.

Duan D, Zheng K, Shen Y, Cao R, Jiang L, Lu Z . Label-free high-throughput microRNA expression profiling from total RNA. Nucleic Acids Res. 2011; 39(22):e154. PMC: 3239174. DOI: 10.1093/nar/gkr774. View

13.

Zhou S, Sha H, Ke X, Liu B, Wang X, Du X . Combination drug release of smart cyclodextrin-gated mesoporous silica nanovehicles. Chem Commun (Camb). 2015; 51(33):7203-6. DOI: 10.1039/c5cc00585j. View

14.

Tu J, Boyle A, Friedrich H, Bomans P, Bussmann J, Sommerdijk N . Mesoporous Silica Nanoparticles with Large Pores for the Encapsulation and Release of Proteins. ACS Appl Mater Interfaces. 2016; 8(47):32211-32219. DOI: 10.1021/acsami.6b11324. View

15.

Climent E, Bernardos A, Martinez-Manez R, Maquieira A, Marcos M, Pastor-Navarro N . Controlled delivery systems using antibody-capped mesoporous nanocontainers. J Am Chem Soc. 2009; 131(39):14075-80. DOI: 10.1021/ja904456d. View

16.

Zhang P, Li Z, Wang H, Zhuo Y, Yuan R, Chai Y . DNA nanomachine-based regenerated sensing platform: a novel electrochemiluminescence resonance energy transfer strategy for ultra-high sensitive detection of microRNA from cancer cells. Nanoscale. 2017; 9(6):2310-2316. DOI: 10.1039/c6nr08631d. View

17.

Lee J, Jung Y . Two-temperature hybridization for microarray detection of label-free microRNAs with attomole detection and superior specificity. Angew Chem Int Ed Engl. 2011; 50(52):12487-90. DOI: 10.1002/anie.201105605. View

18.

Miao X, Cheng Z, Ma H, Li Z, Xue N, Wang P . Label-Free Platform for MicroRNA Detection Based on the Fluorescence Quenching of Positively Charged Gold Nanoparticles to Silver Nanoclusters. Anal Chem. 2017; 90(2):1098-1103. DOI: 10.1021/acs.analchem.7b01991. View

19.

Yan H, Teh C, Sreejith S, Zhu L, Kwok A, Fang W . Functional mesoporous silica nanoparticles for photothermal-controlled drug delivery in vivo. Angew Chem Int Ed Engl. 2012; 51(33):8373-7. DOI: 10.1002/anie.201203993. View

20.

Wang B, Wang H, Yang Z . MiR-122 inhibits cell proliferation and tumorigenesis of breast cancer by targeting IGF1R. PLoS One. 2012; 7(10):e47053. PMC: 3466252. DOI: 10.1371/journal.pone.0047053. View