Molecular Characterization of UFGT Gene and R2R3-MYB Transcription Factor Involved in Flavonoid Biosynthesis in Four Tissues of Wild Pomegranate ( L.)

Overview

Journal J Genet

Specialty Genetics

Date 2019 Nov 27

PMID 31767815

Citations 6

Authors

Ramanjeet Kaur

Nisha Kapoor

Lubna Aslam

Ritu Mahajan

Affiliations

Soon will be listed here.

Abstract

The diversity on fruit colouration in plants directly depends on the flavonoids that explain the development of different pigmentation patterns. Anthocyanins are the major class of flavonoid pigments that are synthesized through flavonoid biosynthetic pathway. In the present study, two genes: UFGT gene and R2R3-MYB gene, involved in anthocyanin biosynthesis were analysed in four tissues of wild pomegranate. The structural genes, UDP-glucose: flavonoid-3-O-glucosyl transferase (UFGT; GenBank accession number: MK058491) and its myeloblastosis transcription factor (R2R3-MYB; GenBank accession number: MK092063) were isolated and their expression pattern were studied. Molecular modelling indicated that the main secondary structures of UFGT and R2R3-MYB genes are α-helix and random coil. In addition, expression profiling of UFGT and R2R3-MYB by quantitative-real time PCR indicated a positive correlation between anthocyanin content and their expression in leaves, flowers, green and red fruits of wild pomegranate. Among all the tissues, the red fruit exhibited high transcripts levels of UFGT as well as R2R3-MYB transcription factor. An extensive homology with UFGTs from other plants was revealed on comparative and bioinformatic analyses. Present study reveals that UFGT plays a predominant role in anthocyanin content in wild pomegranate fruits. Further, it is strongly suggested that R2R3-MYB transcription factor regulates the anthocyanin biosynthesis in wild pomegranate via expression of UFGT gene. This is the first study which provides an insight on expression profile of UFGT and R2R3-MYB that are involved in colour development and fruit ripening in wild pomegranate.

Citing Articles

Genome assembly, annotation and evolutionary insights from the draft genome of wild pomegranate.

Mahajan R, Gupta S, Tanoj N, Sagar T, Kaur S, Hussain S Protoplasma. 2024; .

PMID: 39623009 DOI: 10.1007/s00709-024-02012-2.

Transcriptomic Analysis Reveals the Mechanism of Color Formation in the Peel of an Evergreen Pomegranate Cultivar 'Danruo No.1' During Fruit Development.

Wang X, Yang C, Zhu W, Weng Z, Li F, Teng Y Plants (Basel). 2024; 13(20).

PMID: 39458853 PMC: 11511302. DOI: 10.3390/plants13202903.

A Fruit-Expressed MYB Transcription Factor Regulates Anthocyanin Biosynthesis in .

Liu X, Zhao T, Yuan L, Qiu F, Tang Y, Li D Int J Mol Sci. 2024; 25(9).

PMID: 38732182 PMC: 11084770. DOI: 10.3390/ijms25094963.

Comparative transcriptomic and metabolomic profiles reveal fruit peel color variation in two red pomegranate cultivars.

Zhao X, Feng Y, Ke D, Teng Y, Yuan Z Plant Mol Biol. 2024; 114(3):51.

PMID: 38691187 DOI: 10.1007/s11103-024-01446-9.

The mechanisms underpinning anthocyanin accumulation in a red-skinned bud sport in pear (Pyrus ussuriensis).

Liu W, Wei Y, Sha S, Xu Y, Li H, Yuan H Plant Cell Rep. 2023; 42(6):1089-1105.

PMID: 37062789 DOI: 10.1007/s00299-023-03015-8.

References

Zhang J, Liu Y, Bu Y, Zhang X, Yao Y . Factor Analysis of MYB Gene Expression and Flavonoid Affecting Petal Color in Three Crabapple Cultivars. Front Plant Sci. 2017; 8:137. PMC: 5293739. DOI: 10.3389/fpls.2017.00137. View

Stracke R, WERBER M, Weisshaar B . The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol. 2001; 4(5):447-56. DOI: 10.1016/s1369-5266(00)00199-0. View

Ban Y, Honda C, Hatsuyama Y, Igarashi M, Bessho H, Moriguchi T . Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant Cell Physiol. 2007; 48(7):958-70. DOI: 10.1093/pcp/pcm066. View

Montefiori M, Espley R, Stevenson D, Cooney J, Datson P, Saiz A . Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red-fleshed kiwifruit (Actinidia chinensis). Plant J. 2010; 65(1):106-118. DOI: 10.1111/j.1365-313X.2010.04409.x. View

Li X, Zhang J, Wu Z, Lai B, Huang X, Qin Y . Functional characterization of a glucosyltransferase gene, LcUFGT1, involved in the formation of cyanidin glucoside in the pericarp of Litchi chinensis. Physiol Plant. 2015; 156(2):139-149. DOI: 10.1111/ppl.12391. View

Nakatsuka T, Haruta K, Pitaksutheepong C, Abe Y, Kakizaki Y, Yamamoto K . Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Plant Cell Physiol. 2008; 49(12):1818-29. DOI: 10.1093/pcp/pcn163. View

Shelton D, Stranne M, Mikkelsen L, Pakseresht N, Welham T, Hiraka H . Transcription factors of Lotus: regulation of isoflavonoid biosynthesis requires coordinated changes in transcription factor activity. Plant Physiol. 2012; 159(2):531-47. PMC: 3375922. DOI: 10.1104/pp.112.194753. View

Zhao Z, Hu G, Hu F, Wang H, Yang Z, Lai B . The UDP glucose: flavonoid-3-O-glucosyltransferase (UFGT) gene regulates anthocyanin biosynthesis in litchi (Litchi chinesis Sonn.) during fruit coloration. Mol Biol Rep. 2012; 39(6):6409-15. DOI: 10.1007/s11033-011-1303-3. View

Tan J, Wang M, Tu L, Nie Y, Lin Y, Zhang X . The flavonoid pathway regulates the petal colors of cotton flower. PLoS One. 2013; 8(8):e72364. PMC: 3741151. DOI: 10.1371/journal.pone.0072364. View

10.

Wang H, Jiang Q, Feng X . Effect of Icariin on apoptosis and expression of Fas, Fas ligand, B cell lymphoma, and Bcl-2-associated X protein in CD4+ T lymphocytes from patients with ankylosing spondylitis. J Tradit Chin Med. 2018; 37(2):207-13. DOI: 10.1016/s0254-6272(17)30046-8. View

11.

Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L . MYB transcription factors in Arabidopsis. Trends Plant Sci. 2010; 15(10):573-81. DOI: 10.1016/j.tplants.2010.06.005. View

12.

Gonzalez A, Zhao M, Leavitt J, Lloyd A . Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J. 2007; 53(5):814-27. DOI: 10.1111/j.1365-313X.2007.03373.x. View

13.

Wang Z, Meng D, Wang A, Li T, Jiang S, Cong P . The methylation of the PcMYB10 promoter is associated with green-skinned sport in Max Red Bartlett pear. Plant Physiol. 2013; 162(2):885-96. PMC: 3668077. DOI: 10.1104/pp.113.214700. View

14.

Wen L, Zhao Y, Jiang Y, Yu L, Zeng X, Yang J . Identification of a flavonoid C-glycoside as potent antioxidant. Free Radic Biol Med. 2017; 110:92-101. DOI: 10.1016/j.freeradbiomed.2017.05.027. View

15.

Huang W, Khaldun A, Lv H, Du L, Zhang C, Wang Y . Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum. Plant Cell Rep. 2016; 35(4):883-94. DOI: 10.1007/s00299-015-1929-z. View

16.

Jin H, Martin C . Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol. 2000; 41(5):577-85. DOI: 10.1023/a:1006319732410. View

17.

Xu W, Grain D, Bobet S, Le Gourrierec J, Thevenin J, Kelemen Z . Complexity and robustness of the flavonoid transcriptional regulatory network revealed by comprehensive analyses of MYB-bHLH-WDR complexes and their targets in Arabidopsis seed. New Phytol. 2013; 202(1):132-144. DOI: 10.1111/nph.12620. View

18.

Combet C, Blanchet C, Geourjon C, Deleage G . NPS@: network protein sequence analysis. Trends Biochem Sci. 2000; 25(3):147-50. DOI: 10.1016/s0968-0004(99)01540-6. View

19.

Shaipulah N, Muhlemann J, Woodworth B, Van Moerkercke A, Verdonk J, Ramirez A . CCoAOMT Down-Regulation Activates Anthocyanin Biosynthesis in Petunia. Plant Physiol. 2015; 170(2):717-31. PMC: 4734575. DOI: 10.1104/pp.15.01646. View

20.

Zhou Y, Wu X, Zhang Z, Gao Z . Identification of differentially expressed genes associated with flower color in peach using genome-wide transcriptional analysis. Genet Mol Res. 2015; 14(2):4724-39. DOI: 10.4238/2015.May.11.5. View