Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry

Overview

Journal Anal Chem

Specialty Chemistry

Date 2019 Nov 26

PMID 31765130

Citations 25

Authors

Sarah N Sipe

John W Patrick

Arthur Laganowsky

Jennifer S Brodbelt

Affiliations

Soon will be listed here.

Abstract

Development of chemical chaperones to solubilize membrane protein complexes in aqueous solutions has allowed for gas-phase analysis of their native-like assemblies, including rapid evaluation of stability and interacting partners. Characterization of protein primary sequence, however, has thus far been limited. Ultraviolet photodissociation (UVPD) generates a multitude of sequence ions for the ammonia channel (AmtB), provides improved localization of a possible post-translational modification of aquaporin Z (AqpZ), and surpasses previous reports of sequence coverage for mechanosensitive channel of large conductance (MscL). Variations in UVPD sequence ion abundance have been shown to correspond to structural changes induced upon some perturbation. Preliminary results are reported here for elucidating increased rigidity or flexibility of MscL when bound to various phospholipids.

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