Synergistic Effects and Mechanisms of Combined Treatment With Harmine Hydrochloride and Azoles for Resistant

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Nov 22

PMID 31749766

Citations 8

Authors

Xiuyun Li

Xuexin Wu

Yan Gao

Lina Hao

Affiliations

Soon will be listed here.

Abstract

Several studies have demonstrated the significant antiviral, antimicrobial, antiplasmodial, antioxidative, antifungal, antimutagenic, and antitumor properties of harmine hydrochloride (HMH). The main objective of the present study was to investigate the antifungal effects and underlying mechanisms of HMH when combined with azoles to determine whether such combinations act in a synergistic manner. As a result, we found that HMH exhibits synergistic antifungal effects in combination with azoles against resistant () planktonic cells, as well as resistant biofilm in the early stage. Antifungal potential of HMH with fluconazole was also explored using an invertebrate model. Our results suggest that HMH combined with azoles is synergistic against resistant and . No synergy is seen with azole sensitive strains nor with other species. Such synergistic mechanisms on resistance are involved in increasing intracellular azoles, inhibiting hyphal growth, disturbing cytosolic calcium concentration, and inducing apoptosis of resistant cells.

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