Transcriptome Analysis of Choke Stroma and Asymptomatic Inflorescence Tissues Reveals Changes in Gene Expression in Both and Its Host Plant Subsp.

Overview

Journal Microorganisms

Specialty Microbiology

Date 2019 Nov 21

PMID 31744076

Citations 5

Authors

Ruying Wang

Bruce B Clarke

Faith C Belanger

Affiliations

Soon will be listed here.

Abstract

Many cool-season grasses have symbiotic relationships with (Ascomycota, Clavicipitaceae) fungal endophytes that inhabit the intercellular spaces of the above-ground parts of the host plants. The presence of the endophytes is generally beneficial to the hosts due to enhanced tolerance to biotic and abiotic stresses conferred by the endophytes. Many spp. are asexual, and those infections always remain asymptomatic. However, some spp. have a sexual stage and produce a macroscopic fruiting body, a stroma, that envelops the developing inflorescence causing a syndrome termed "choke disease". Here, we report a fungal and plant gene expression analysis of choke stroma tissue and asymptomatic inflorescence tissue of -infected strong creeping red fescue ( subsp. ). Hundreds of fungal genes and over 10% of the plant genes were differentially expressed when comparing the two tissue types. The differentially expressed fungal genes in the choke stroma tissue indicated a change in carbohydrate and lipid metabolism, as well as a change in expression of numerous genes for candidate effector proteins. Plant stress-related genes were up-regulated in the stroma tissue, suggesting the plant host was responding to the epiphytic stage of as a pathogen.

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