Molecular Epidemiology of and Its Relationship with the Occurrence of Necrotizing Enterocolitis in Preterm Neonates

Overview

Journal New Microbes New Infect

Publisher Elsevier

Specialty Infectious Diseases

Date 2019 Nov 19

PMID 31737279

Citations 10

Authors

M Hosny

E Baptiste

A Levasseur

B La Scola

Affiliations

Soon will be listed here.

Abstract

Clostridia-especially -are among the taxa most frequently identified from stool samples of preterm neonates with necrotizing enterocolitis (NEC). Recently, has also been detected from epidemic cases, but using a culture-based approach we were unable to confirm this discovery in a local cohort. In order to investigate this link by a molecular approach, a specific -based quantitative real-time PCR was developed to detect directly from patients' stool specimens. Design of this -based quantitative real-time PCR was based on the genomic analysis of seven clinical isolates of . It was tested on stool samples from 88 preterm neonates with necrotizing enterocolitis and 71 matched controls. was significantly more prevalent in stools from preterm neonates with necrotizing enterocolitis than in controls (respectively 30/88 (34%) versus 9/71 (13%); p 0.003). Whole-genome analysis also allowed the identification of three genomic clusters of . This clustering was associated with a geographical location regardless of isolation from the NEC or control, suggesting asymptomatic carriage. Although less prevalent than in our cohort, is significantly associated with the occurrence of necrotizing enterocolitis.

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