Identification of Species from Clinical Samples in a Honduran Tertiary Hospital

Overview

Journal Pathogens

Date 2019 Nov 17

PMID 31731617

Citations 12

Authors

Kathy Montes

Bryan Ortiz

Celeste Galindo

Isis Figueroa

Sharleen Braham

Gustavo Fontecha

Affiliations

Soon will be listed here.

Abstract

species are one of the most important causes of human infections, especially in hospitals and among immunocompromised patients. The correct and rapid etiological identification of yeast infections is important to provide adequate therapy, reduce mortality, and control outbreaks. In this study, species were identified in patients with suspected fungal infection, and phenotypic and genotypic identification methods were compared. A total of 167 axenic fungal cultures and 46 clinical samples were analyzed by HardyCHROM, MicroScan(Omron Microscan Systems Inc, Renton, WA, USA), and PCR-RFLP (Restriction Fragment Length Polymorphisms). The species of the complex were the most frequent, followed by and . Less common but clinically relevant species of were also isolated. The comparison between the three methods was concordant, especially for the most common species. Fungal DNA amplification was successful in all clinical samples.

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