Systematic Analysis of Alkaline/Neutral Invertase Genes Reveals the Involvement of Smi-miR399 in Regulation of and in

Overview

Journal Plants (Basel)

Date 2019 Nov 14

PMID 31717988

Citations 4

Authors

Hong Zhou

Caili Li

Xiaoxiao Qiu

Shanfa Lu

Affiliations

Soon will be listed here.

Abstract

Alkaline/neutral invertases (), which irreversibly catalyze the hydrolysis of sucrose into fructose and glucose, play crucial roles in carbohydrate metabolism and plant development. Comprehensive insights into genes are lacking in , a well-known traditional Chinese medicinal (TCM) plant with significant medicinal and economic value. Through genome-wide prediction, nine putative genes, termed -, were identified. Integrated analysis of gene structures, sequence features, conserved domains, conserved motifs and phylogenetic trees revealed the conservation and divergence of . The identified were differentially expressed in roots, stems, leaves, flowers, and different root tissues. They also responded to drought, salicylic acid, yeast extract, and methyl jasmonate treatments. More importantly, computational prediction and experimental validation showed that and were targets of Smi-miR399, a conserved miRNA previously shown to affect Pi uptake and translocation through the cleavage of (). Consistently, analysis of 43 genes and 26 miR399 sequences from , , and showed that various , , and genes were regulated by miR399. It indicates that the miR399- module exists widely in plants. Furthermore, Smi-miR399 also cleaved transcripts in , suggesting the complexity of , and miR399 networks.

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