Anti-Osteoporotic Effects of Combined Extract of and in Osteoblast and Osteoclast Cells and Ovariectomized Mice

Overview

Journal Nutrients

Date 2019 Nov 14

PMID 31717518

Citations 10

Authors

Eunkuk Park

Jeonghyun Kim

Subin Yeo

Eunguk Lim

Chun Whan Choi

Sangho Choi

Wan Yi Li

Ji-Won Lee

Jin-Hyok Park

Dam Huh

Seon-Yong Jeong

Affiliations

Soon will be listed here.

Abstract

Osteoporosis is characterized by low bone density and quality with high risk of bone fracture. Here, we investigated anti-osteoporotic effects of natural plants ( (LRC) and (AJ)) in osteoblast and osteoclast cells in vitro and ovariectomized mice in vivo. Combined LRC and AJ enhanced osteoblast differentiation and mineralized bone-forming osteoblasts by the up-regulation of bone metabolic markers ( and ) in the osteoblastic cell line MC3T3-E1. However, LRC and AJ inhibited osteoclast differentiation of monocytes isolated from mouse bone marrow. In vivo experiments showed that treatment of LRC+AJ extract prevented OVX-induced trabecular bone loss and osteoclastogenesis in an osteoporotic animal model. These results suggest that LRC+AJ extract may be a good therapeutic agent for the treatment and prevention of osteoporotic bone loss.

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References

Liu J, Kyung Nam H, Campbell C, Gasque K, Millan J, Hatch N . Tissue-nonspecific alkaline phosphatase deficiency causes abnormal craniofacial bone development in the Alpl(-/-) mouse model of infantile hypophosphatasia. Bone. 2014; 67:81-94. PMC: 4149826. DOI: 10.1016/j.bone.2014.06.040. View

Hattersley G, Chambers T . Generation of osteoclastic function in mouse bone marrow cultures: multinuclearity and tartrate-resistant acid phosphatase are unreliable markers for osteoclastic differentiation. Endocrinology. 1989; 124(4):1689-96. DOI: 10.1210/endo-124-4-1689. View

Boyle W, Simonet W, Lacey D . Osteoclast differentiation and activation. Nature. 2003; 423(6937):337-42. DOI: 10.1038/nature01658. View

Willett T, Pasquale J, Grynpas M . Collagen modifications in postmenopausal osteoporosis: advanced glycation endproducts may affect bone volume, structure and quality. Curr Osteoporos Rep. 2014; 12(3):329-37. DOI: 10.1007/s11914-014-0214-3. View

Cappola A, Shoback D . Osteoporosis Therapy in Postmenopausal Women With High Risk of Fracture. JAMA. 2016; 316(7):715-6. DOI: 10.1001/jama.2016.11032. View

Simonet W, Lacey D, Dunstan C, Kelley M, Chang M, Luthy R . Osteoprotegerin: a novel secreted protein involved in the regulation of bone density. Cell. 1997; 89(2):309-19. DOI: 10.1016/s0092-8674(00)80209-3. View

He L, Lee J, Jang J, Sakchaisri K, Hwang J, Cha-Molstad H . Osteoporosis regulation by salubrinal through eIF2α mediated differentiation of osteoclast and osteoblast. Cell Signal. 2012; 25(2):552-60. PMC: 3593652. DOI: 10.1016/j.cellsig.2012.11.015. View

Chou J, Hao J, Hatoyama H, Ben-Nissan B, Milthorpe B, Otsuka M . The therapeutic effect on bone mineral formation from biomimetic zinc containing tricalcium phosphate (ZnTCP) in zinc-deficient osteoporotic mice. PLoS One. 2013; 8(8):e71821. PMC: 3742499. DOI: 10.1371/journal.pone.0071821. View

Watts N . Clinical utility of biochemical markers of bone remodeling. Clin Chem. 1999; 45(8 Pt 2):1359-68. View

10.

Bernhardt A, Schamel M, Gbureck U, Gelinsky M . Osteoclastic differentiation and resorption is modulated by bioactive metal ions Co2+, Cu2+ and Cr3+ incorporated into calcium phosphate bone cements. PLoS One. 2017; 12(8):e0182109. PMC: 5538673. DOI: 10.1371/journal.pone.0182109. View

11.

Bonura F . Prevention, screening, and management of osteoporosis: an overview of the current strategies. Postgrad Med. 2009; 121(4):5-17. DOI: 10.3810/pgm.2009.07.2021. View

12.

Kim J, Lee S, Kim S, Na S, Kim Y . Osteoprotective effect of extract from Achyranthes japonica in ovariectomized rats. J Exerc Rehabil. 2015; 10(6):372-7. PMC: 4294440. DOI: 10.12965/jer.140169. View

13.

Park E, Jin H, Cho D, Kim J, Kim M, Choi C . The effect of Lycii Radicis Cortex extract on bone formation in vitro and in vivo. Molecules. 2014; 19(12):19594-609. PMC: 6271141. DOI: 10.3390/molecules191219594. View

14.

Lee N, Sowa H, Hinoi E, Ferron M, Ahn J, Confavreux C . Endocrine regulation of energy metabolism by the skeleton. Cell. 2007; 130(3):456-69. PMC: 2013746. DOI: 10.1016/j.cell.2007.05.047. View

15.

Yuan H, Ma Q, Ye L, Piao G . The Traditional Medicine and Modern Medicine from Natural Products. Molecules. 2016; 21(5). PMC: 6273146. DOI: 10.3390/molecules21050559. View

16.

Ominsky M, Li X, Asuncion F, Barrero M, Warmington K, Dwyer D . RANKL inhibition with osteoprotegerin increases bone strength by improving cortical and trabecular bone architecture in ovariectomized rats. J Bone Miner Res. 2008; 23(5):672-82. DOI: 10.1359/jbmr.080109. View

17.

Zheng M, Ge Y, Li H, Yan M, Zhou J, Zhang Y . Bergapten prevents lipopolysaccharide mediated osteoclast formation, bone resorption and osteoclast survival. Int Orthop. 2013; 38(3):627-34. PMC: 3936084. DOI: 10.1007/s00264-013-2184-y. View

18.

Barnes J, McLachlan A, Mt Sherwin C, Enioutina E . Herbal medicines: challenges in the modern world. Part 1. Australia and New Zealand. Expert Rev Clin Pharmacol. 2016; 9(7):905-15. DOI: 10.1586/17512433.2016.1171712. View

19.

Gilbert L, He X, Farmer P, Rubin J, Drissi H, van Wijnen A . Expression of the osteoblast differentiation factor RUNX2 (Cbfa1/AML3/Pebp2alpha A) is inhibited by tumor necrosis factor-alpha. J Biol Chem. 2001; 277(4):2695-701. DOI: 10.1074/jbc.M106339200. View

20.

Teitelbaum S . Bone resorption by osteoclasts. Science. 2000; 289(5484):1504-8. DOI: 10.1126/science.289.5484.1504. View