Characterization of Alpha-1-antitrypsin by Isoelectric Focusing on an Ultrathin Polyacrylamide Gel Layer. An Economic High-resolution System for Determining PiM Subtypes

Overview

Journal Hum Genet

Specialty Genetics

Date 1979 Jan 1

PMID 317068

Citations 5

Authors

G Massi

A Fabiano

D Ragusa

P Auconi

Affiliations

Soon will be listed here.

Abstract

Through the use of ultrathin layer polyacrylamide gel isoelectric focusing it is possible to obtain a resolution of the bands of alpha 1AT so as to be able to easily recognize all six PiM subtypes. The optimal resolution of the PiM subtypes is obtained without deforming the pattern of the Pi phenotypes. In addition to high resolution, ultrathin layer polyacrylamide gel isoelectric focusing permits a notable reduction of fees to 1/5 of the usual.

Citing Articles

Rapid and inexpensive detection of alpha1-antitrypsin deficiency-related alleles S and Z by a real-time polymerase chain reaction suitable for a large-scale population-based screening.

Kaczor M, Sanak M, Szczeklik A J Mol Diagn. 2007; 9(1):99-104.

PMID: 17251342 PMC: 1867421. DOI: 10.2353/jmoldx.2007.060048.

Alpha-1-antitrypsin phenotypes in malignant lymphoma.

Callea F, Massi G, De Wolf-Peeters C, Lievens C, Desmet V J Clin Pathol. 1982; 35(11):1213-5.

PMID: 6982905 PMC: 497929. DOI: 10.1136/jcp.35.11.1213.

Alpha-1-antitrypsin (alpha 1AT) phenotypes and PiM subtypes in Italy. Evidence of considerable geographic variability.

Massi G, Cotumaccio R, Auconi P Hum Genet. 1982; 61(1):76-7.

PMID: 6982216 DOI: 10.1007/BF00291340.

Pi M4: an additional Pi M subtype.

Constans J, Viau M, Gouaillard C Hum Genet. 1980; 55(1):119-21.

PMID: 6969686 DOI: 10.1007/BF00329137.

Genetic regulation of alpha-1-antitrypsin levels in the neonatal period: the influence of the PiS allele and the PiM subtypes.

Auconi P, Biagini R, DONNINI R, Fabiano A, Ragusa D, Massi G Eur J Pediatr. 1981; 136(2):199-202.

PMID: 6164556 DOI: 10.1007/BF00441924.

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