Transcriptome Profiling of 'Kyoho' Grape at Different Stages of Berry Development Following 5-azaC Treatment

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2019 Nov 10

PMID 31703618

Citations 3

Authors

Da-Long Guo

Qiong Li

Xiao-Ru Ji

Zhen-Guang Wang

Yi-He Yu

Affiliations

Soon will be listed here.

Abstract

Background: 5-Azacytidine (5-azaC) promotes the development of 'Kyoho' grape berry but the associated changes in gene expression have not been reported. In this study, we performed transcriptome analysis of grape berry at five developmental stages after 5-azaC treatment to elucidate the gene expression networks controlling berry ripening.

Results: The expression patterns of most genes across the time series were similar between the 5-azaC treatment and control groups. The number of differentially expressed genes (DEGs) at a given developmental stage ranged from 9 (A3_C3) to 690 (A5_C5). The results indicated that 5-azaC treatment had not very great influences on the expressions of most genes. Functional annotation of the DEGs revealed that they were mainly related to fruit softening, photosynthesis, protein phosphorylation, and heat stress. Eight modules showed high correlation with specific developmental stages and hub genes such as PEROXIDASE 4, CAFFEIC ACID 3-O-METHYLTRANSFERASE 1, and HISTONE-LYSINE N-METHYLTRANSFERASE EZA1 were identified by weighted gene correlation network analysis.

Conclusions: 5-AzaC treatment alters the transcriptional profile of grape berry at different stages of development, which may involve changes in DNA methylation.

Citing Articles

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Azacytidine arrests ripening in cultivated strawberry (Fragaria × ananassa) by repressing key genes and altering hormone contents.

Martinez-Rivas F, Blanco-Portales R, Molina-Hidalgo F, Caballero J, de Souza L, Alseekh S BMC Plant Biol. 2022; 22(1):278.

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Demethylation alters transcriptome profiling of buds and leaves in 'Kyoho' grape.

Jia H, Zhang Z, Sadeghnezhad E, Pang Q, Li S, Pervaiz T BMC Plant Biol. 2020; 20(1):544.

PMID: 33276735 PMC: 7716455. DOI: 10.1186/s12870-020-02754-0.

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