A Nanocomposite Prepared from Platinum Particles, Polyaniline and a TiC MXene for Amperometric Sensing of Hydrogen Peroxide and Lactate

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2019 Nov 9

PMID 31701355

Citations 12

Authors

Supawat Neampet

Nipapan Ruecha

Jiaqian Qin

Wanida Wonsawat

Orawon Chailapakul

Nadnudda Rodthongkum

Affiliations

Soon will be listed here.

Abstract

A nanocomposite consisting of platinum particles, polyaniline and TiC MXene (Pt/PANI/MXene) was used to modify a screen-printed carbon electrode (SPCE) to obtain sensors for hydrogen peroxide and lactate. This nanocomposite was characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM) and X-ray powder diffraction (XRD) to determine the physical morphologies and the nanocomposite elements. The modified electrode exhibited the improved current response towards hydrogen peroxide (HO) compared with an unmodified electrode and provided a low detection limit of 1.0 μM. When lactate oxidase was immobilized on the modified electrode, the electrode responded to lactate via the HO generated in the enzymatic reaction. The lactate assay was performed by amperometry at a constant potential of +0.3 V (vs. Ag/AgCl). The linear range was found to be from 0.005 to 5.0 mM with a detection limit of 5.0 μM for lactate. Ultimately, this biosensor was used for the determination of lactate in milk samples with high stability and reliability. Graphical abstractSchematic representation of a novel platinum particles/polyaniline/MXene nanocomposite (Pt/PANI/MXene) for screen-printed carbon electrode (SPCE) modification to enhance the specific surface area for immobilization of lactate oxidase (LOx) and use as enzymatic biosensor for lactate determination in milk sample.

Citing Articles

A Comprehensive Review of Advanced Lactate Biosensor Materials, Methods, and Applications in Modern Healthcare.

Ding Y, Yang L, Wen J, Ma Y, Dai G, Mo F Sensors (Basel). 2025; 25(4).

PMID: 40006275 PMC: 11858931. DOI: 10.3390/s25041045.

Development of electrochemical sensors for quick detection of environmental (soil and water) NPK ions.

Hossain M, Khaleque M, Ali M, Bacchu M, Hossain M, Shahed S RSC Adv. 2024; 14(13):9137-9158.

PMID: 38505387 PMC: 10949039. DOI: 10.1039/d4ra00034j.

Disposable Polyaniline/-Phenylenediamine-Based Electrochemical Lactate Biosensor for Early Sepsis Diagnosis.

Thongkhao P, Numnuam A, Khongkow P, Sangkhathat S, Phairatana T Polymers (Basel). 2024; 16(4).

PMID: 38399851 PMC: 10892424. DOI: 10.3390/polym16040473.

TiC-MXene/NiO Nanocomposites-Decorated CsPbI Perovskite Active Materials under UV-Light Irradiation for the Enhancement of Crystal-Violet Dye Photodegradation.

Alothman A, Khan M, Albaqami M, Mohandoss S, ALOthman Z, Ahmad N Nanomaterials (Basel). 2023; 13(23).

PMID: 38063722 PMC: 10707859. DOI: 10.3390/nano13233026.

Titanium Carbide (TiCT) MXene as Efficient Electron/Hole Transport Material for Perovskite Solar Cells and Electrode Material for Electrochemical Biosensors/Non-Biosensors Applications.

Niyitanga T, Chaudhary A, Ahmad K, Kim H Micromachines (Basel). 2023; 14(10).

PMID: 37893344 PMC: 10609296. DOI: 10.3390/mi14101907.

References

Crespilho F, Iost R, Travain S, Oliveira Jr O, Zucolotto V . Enzyme immobilization on Ag nanoparticles/polyaniline nanocomposites. Biosens Bioelectron. 2009; 24(10):3073-7. DOI: 10.1016/j.bios.2009.03.026. View

Shimomura T, Sumiya T, Ono M, Ito T, Hanaoka T . Amperometric L-lactate biosensor based on screen-printed carbon electrode containing cobalt phthalocyanine, coated with lactate oxidase-mesoporous silica conjugate layer. Anal Chim Acta. 2012; 714:114-20. DOI: 10.1016/j.aca.2011.11.053. View

Rakhi R, Nayak P, Xia C, Alshareef H . Novel amperometric glucose biosensor based on MXene nanocomposite. Sci Rep. 2016; 6:36422. PMC: 5103228. DOI: 10.1038/srep36422. View

Wang F, Yang C, Duan M, Tang Y, Zhu J . TiO2 nanoparticle modified organ-like Ti3C2 MXene nanocomposite encapsulating hemoglobin for a mediator-free biosensor with excellent performances. Biosens Bioelectron. 2015; 74:1022-8. DOI: 10.1016/j.bios.2015.08.004. View

Yu X, Li Y, Cheng J, Liu Z, Li Q, Li W . Monolayer Ti₂CO₂: A Promising Candidate for NH₃ Sensor or Capturer with High Sensitivity and Selectivity. ACS Appl Mater Interfaces. 2015; 7(24):13707-13. DOI: 10.1021/acsami.5b03737. View

Hernandez-Ibanez N, Garcia-Cruz L, Montiel V, Foster C, Banks C, Iniesta J . Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures. Biosens Bioelectron. 2015; 77:1168-74. DOI: 10.1016/j.bios.2015.11.005. View

Male K, Hrapovic S, Luong J . Electrochemically-assisted deposition of oxidases on platinum nanoparticle/multi-walled carbon nanotube-modified electrodes. Analyst. 2008; 132(12):1254-61. DOI: 10.1039/b712478c. View

Wonsawat W, Chuanuwatanakul S, Dungchai W, Punrat E, Motomizu S, Chailapakul O . Graphene-carbon paste electrode for cadmium and lead ion monitoring in a flow-based system. Talanta. 2012; 100:282-9. DOI: 10.1016/j.talanta.2012.07.045. View

Parra-Alfambra A, Casero E, Petit-Dominguez M, Barbadillo M, Pariente F, Vazquez L . New nanostructured electrochemical biosensors based on three-dimensional (3-mercaptopropyl)-trimethoxysilane network. Analyst. 2010; 136(2):340-7. DOI: 10.1039/c0an00475h. View

10.

Kumar S, Lei Y, Alshareef N, Quevedo-Lopez M, Salama K . Biofunctionalized two-dimensional TiC MXenes for ultrasensitive detection of cancer biomarker. Biosens Bioelectron. 2018; 121:243-249. DOI: 10.1016/j.bios.2018.08.076. View

11.

Yukird J, Wongtangprasert T, Rangkupan R, Chailapakul O, Pisitkun T, Rodthongkum N . Label-free immunosensor based on graphene/polyaniline nanocomposite for neutrophil gelatinase-associated lipocalin detection. Biosens Bioelectron. 2016; 87:249-255. DOI: 10.1016/j.bios.2016.08.062. View

12.

Wu L, Lu X, Dhanjai , Wu Z, Dong Y, Wang X . 2D transition metal carbide MXene as a robust biosensing platform for enzyme immobilization and ultrasensitive detection of phenol. Biosens Bioelectron. 2018; 107:69-75. DOI: 10.1016/j.bios.2018.02.021. View

13.

Lamas-Ardisana P, Loaiza O, Anorga L, Jubete E, Borghei M, Ruiz V . Disposable amperometric biosensor based on lactate oxidase immobilised on platinum nanoparticle-decorated carbon nanofiber and poly(diallyldimethylammonium chloride) films. Biosens Bioelectron. 2014; 56:345-51. DOI: 10.1016/j.bios.2014.01.047. View

14.

Kim S, Koh H, Ren C, Kwon O, Maleski K, Cho S . Metallic TiCT MXene Gas Sensors with Ultrahigh Signal-to-Noise Ratio. ACS Nano. 2018; 12(2):986-993. DOI: 10.1021/acsnano.7b07460. View

15.

Loaiza O, Lamas-Ardisana P, Anorga L, Jubete E, Ruiz V, Borghei M . Graphitized carbon nanofiber-Pt nanoparticle hybrids as sensitive tool for preparation of screen printing biosensors. Detection of lactate in wines and ciders. Bioelectrochemistry. 2014; 101:58-65. DOI: 10.1016/j.bioelechem.2014.07.005. View

16.

Lorencova L, Bertok T, Dosekova E, Holazova A, Paprckova D, Vikartovska A . Electrochemical performance of TiCT MXene in aqueous media: towards ultrasensitive H2O2 sensing. Electrochim Acta. 2017; 235:471-479. PMC: 5669457. DOI: 10.1016/j.electacta.2017.03.073. View

17.

Xu B, Zhu M, Zhang W, Zhen X, Pei Z, Xue Q . Ultrathin MXene-Micropattern-Based Field-Effect Transistor for Probing Neural Activity. Adv Mater. 2016; 28(17):3333-9. DOI: 10.1002/adma.201504657. View