Catalytides Derived from the Box A Region in the ANA/BTG3 Protein Cleave Amyloid-β Fragment Peptide

Overview

Journal Heliyon

Specialty Social Sciences

Date 2019 Nov 6

PMID 31687556

Citations 8

Authors

Yusuke Hatakawa

Rina Nakamura

Motomi Konishi

Toshiyasu Sakane

Motoaki Saito

Toshifumi Akizawa

Affiliations

Soon will be listed here.

Abstract

We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.

Citing Articles

An 11-mer Synthetic Peptide Suppressing Aggregation of Aβ25-35 and Resolving Its Aggregated Form Improves Test Performance in an Aβ25-35-Induced Alzheimer's Mouse Model.

Nakamura R, Matsuda A, Higashi Y, Hayashi Y, Konishi M, Saito M Biomolecules. 2024; 14(10).

PMID: 39456166 PMC: 11506537. DOI: 10.3390/biom14101234.

SKGQA, a Peptide Derived from the ANA/BTG3 Protein, Cleaves Amyloid-β with Proteolytic Activity.

Hatakawa Y, Nakamura R, Akizawa T, Konishi M, Matsuda A, Oe T Biomolecules. 2024; 14(5).

PMID: 38785993 PMC: 11118129. DOI: 10.3390/biom14050586.

Cascade autohydrolysis of Alzheimer's Aβ peptides.

Wolfram M, Tiwari M, Hassenkam T, Li M, Bjerrum M, Meldal M Chem Sci. 2023; 14(19):4986-4996.

PMID: 37206405 PMC: 10189894. DOI: 10.1039/d2sc06668h.

Five-mer peptides prevent short-term spatial memory deficits in Aβ25-35-induced Alzheimer's model mouse by suppressing Aβ25-35 aggregation and resolving its aggregate form.

Nakamura R, Konishi M, Higashi Y, Saito M, Akizawa T Alzheimers Res Ther. 2023; 15(1):83.

PMID: 37076912 PMC: 10114458. DOI: 10.1186/s13195-023-01229-2.

Structure-Activity Relationship of 5-mer Catalytides, GSGYR and RYGSG.

Nakamura R, Akizawa T, Konishi M Biomolecules. 2022; 12(12).

PMID: 36551193 PMC: 9775622. DOI: 10.3390/biom12121766.

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