Positive Reinforcement-Based Training for Self-Loading of Meat Horses Reduces Loading Time and Stress-Related Behavior

The present work aimed to evaluate the efficacy of a self-loading training using positive reinforcement on stress-related behaviors shown by meat horses during loading procedures into a truck. Thirty-two meat horses ( = 18; = 14; 6 month-old) were included in the study. All horses had limited interactions with the farmer and were not used to be restrained nor lead by halter. Horses were divided in two groups: Control Group (C; = 14) and Training Group (T; = 18). T horses were trained to self-load: in order to teach the horses to enter into the truck, a targeting training technique throughout a shaping process was applied. Training sessions were performed three times a week, from 9:30 a.m. to 1:30 p.m. and from 2:30 p.m. to 4:30 p.m., for 6 weeks; training was then repeated once a week to maintain the memory until the transport toward a slaughterhouse. The loading phase was video-recorded and loading time was directly recorded using a stopwatch. All horses were transported to the same slaughterhouse in 14 different days using the same truck. Behavior was subsequently analyzed with a focal animal continuous recording method. Loading time was shorter in T horses (mean ± SD = 44.44 ± 47.58 s) than in C horses (mean ± SD = 463.09 ± 918.19 s) (-test; = 0.019). T horses showed more forward locomotion toward the truck than C horses (-test; = 0.029). Our preliminary findings suggest that self-loading training may be useful to mitigate loading-related stress in meat horses, minimally socialized with humans.